Administration of alpha klotho reduces liver and adipose lipid accumulation in obese mice
Rao, Zhijian; Landry, Taylor; Li, Peixin; Bunner, Wyatt; Laing, Brenton Thomas; Yuan, Yuan; Huang, Hu
α-Klotho, a known anti-aging protein, exerts diverse physiological effects including: maintenance of phosphate and calcium homeostasis, modulation of cell proliferation, and enhanced buffering of reactive oxygen species. However, the role of α-Klotho in the regulation of energy metabolism is complex and poorly understood. Here we investigated the effects of 5 weeks peripheral administration of α-Klotho in high fat diet induced obese mice. Food intake, blood glucose, and body weight were measured daily. Energy expenditure was determined with indirect calorimetry and body composition with magnetic resonance imaging. Liver and adipose tissue were collected for lipid content measurements and gene expression analysis. α-Klotho-treated mice experienced reduced adiposity, increased lean mass, and elevated energy expenditure, despite no changes in food intake, body weight, or fed blood glucose levels. Lipid accumulation in liver and adipose tissue was also reduced compared to controls. Furthermore, Real-time quantitative PCR showed reduced expression of key lipogenic genes in α-Klotho treated mice in these organs. Taken together, these data suggest encouraging therapeutic potential of α-Klotho and highlight a need for further research into the specific mechanisms explaining improved body composition, elevated energy expenditure, and reduced lipid content in both liver and adipose tissue in α-Klotho-treated mice.
Rao, Zhijian, & Landry, Taylor, & Li, Peixin, & Bunner, Wyatt, & Laing, Brenton Thomas, & Yuan, Yuan, & Huang, Hu. (April 2019). Administration of alpha klotho reduces liver and adipose lipid accumulation in obese mice. Heliyon, (. Retrieved from http://hdl.handle.net/10342/7840
Rao, Zhijian, and Landry, Taylor, and Li, Peixin, and Bunner, Wyatt, and Laing, Brenton Thomas, and Yuan, Yuan, and Huang, Hu. "Administration of alpha klotho reduces liver and adipose lipid accumulation in obese mice". Heliyon. . (.), April 2019. October 30, 2020. http://hdl.handle.net/10342/7840.
Rao, Zhijian and Landry, Taylor and Li, Peixin and Bunner, Wyatt and Laing, Brenton Thomas and Yuan, Yuan and Huang, Hu, "Administration of alpha klotho reduces liver and adipose lipid accumulation in obese mice," Heliyon 5, no. (April 2019), http://hdl.handle.net/10342/7840 (accessed October 30, 2020).
Rao, Zhijian, Landry, Taylor, Li, Peixin, Bunner, Wyatt, Laing, Brenton Thomas, Yuan, Yuan, Huang, Hu. Administration of alpha klotho reduces liver and adipose lipid accumulation in obese mice. Heliyon. April 2019; 5() . http://hdl.handle.net/10342/7840. Accessed October 30, 2020.