Early cell loss associated with mesenchymal stem cell cardiomyoplasty
Author
Collins, Maria C.; Moore, Joel L. Jr; Burrows, Brian J.; Kypson, Alan P.; Muller-Borer, Barbara J.
Abstract
Background: Human mesenchymal stem cells (hMSCs) show potential for therapeutic cellular cardiomyoplasty. However, a range of delivery methods, including direct intramyocardial injection, have resulted in poor engraftment in vivo. We used the in vivo rat heart model to study hMSC engraftment and retention in a normal beating heart. Materials and Methods: HMSCs transfected with green fluorescent protein were injected into the left ventricle (LV) of immunocompetent rats. Hearts were cryopreserved 30 minutes (Group A), 24 hours (Group B), and 5 days (Group C) post hMSC delivery. HMSC retention was estimated using confocal fluorescence microscopy and immunohistochemistry. Measured values were compared to projected GFP-positive cellular volumes. Immunohistochemical analyses probed for the presence of human cells with human prolyl hydroxylase beta (p4hβ) and an immune response with murine monocyte/macrophage antigen (CD68). Results: HMSC retention decreased significantly from 30 minutes to 5 days
(p<0.05). In Group A the projected GFP positive cellular volume of 31% correlated with measured values and was significantly greater than the 1% predicted cellular volume in Group C. Moreover, human p4hβ was detected in Groups A and B, and not in Group C. Conversely, CD68 was detected in Groups B and C and not in Group A. Conclusions: In immunocompetent rats, engraftment and retention of hMSCs delivered intramyocardially significantly declines over a five day period. The influx of monocytes/macrophages suggests an unfavorable micro-environment for exogenous stem cell survival, confirmed by the absence of human cells detected five days post injection.
Date
2012-03-01
Citation:
APA:
Collins, Maria C., & Moore, Joel L. Jr, & Burrows, Brian J., & Kypson, Alan P., & Muller-Borer, Barbara J.. (March 2012).
Early cell loss associated with mesenchymal stem cell cardiomyoplasty.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/7860
MLA:
Collins, Maria C., and Moore, Joel L. Jr, and Burrows, Brian J., and Kypson, Alan P., and Muller-Borer, Barbara J..
"Early cell loss associated with mesenchymal stem cell cardiomyoplasty". .
. (),
March 2012.
September 22, 2023.
http://hdl.handle.net/10342/7860.
Chicago:
Collins, Maria C. and Moore, Joel L. Jr and Burrows, Brian J. and Kypson, Alan P. and Muller-Borer, Barbara J.,
"Early cell loss associated with mesenchymal stem cell cardiomyoplasty," , no.
(March 2012),
http://hdl.handle.net/10342/7860 (accessed
September 22, 2023).
AMA:
Collins, Maria C., Moore, Joel L. Jr, Burrows, Brian J., Kypson, Alan P., Muller-Borer, Barbara J..
Early cell loss associated with mesenchymal stem cell cardiomyoplasty. .
March 2012;
():
.
http://hdl.handle.net/10342/7860. Accessed
September 22, 2023.
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