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    Early cell loss associated with mesenchymal stem cell cardiomyoplasty

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    Author
    Collins, Maria C.; Moore, Joel L. Jr; Burrows, Brian J.; Kypson, Alan P.; Muller-Borer, Barbara J.
    Abstract
    Background: Human mesenchymal stem cells (hMSCs) show potential for therapeutic cellular cardiomyoplasty. However, a range of delivery methods, including direct intramyocardial injection, have resulted in poor engraftment in vivo. We used the in vivo rat heart model to study hMSC engraftment and retention in a normal beating heart. Materials and Methods: HMSCs transfected with green fluorescent protein were injected into the left ventricle (LV) of immunocompetent rats. Hearts were cryopreserved 30 minutes (Group A), 24 hours (Group B), and 5 days (Group C) post hMSC delivery. HMSC retention was estimated using confocal fluorescence microscopy and immunohistochemistry. Measured values were compared to projected GFP-positive cellular volumes. Immunohistochemical analyses probed for the presence of human cells with human prolyl hydroxylase beta (p4hβ) and an immune response with murine monocyte/macrophage antigen (CD68). Results: HMSC retention decreased significantly from 30 minutes to 5 days (p<0.05). In Group A the projected GFP positive cellular volume of 31% correlated with measured values and was significantly greater than the 1% predicted cellular volume in Group C. Moreover, human p4hβ was detected in Groups A and B, and not in Group C. Conversely, CD68 was detected in Groups B and C and not in Group A. Conclusions: In immunocompetent rats, engraftment and retention of hMSCs delivered intramyocardially significantly declines over a five day period. The influx of monocytes/macrophages suggests an unfavorable micro-environment for exogenous stem cell survival, confirmed by the absence of human cells detected five days post injection.
    URI
    http://hdl.handle.net/10342/7860
    Subject
    Mesenchymal stem cells, cardiomyoplasty, engraftment, intramyocardial injection, cardiac
    Date
    2012-03-01
    Citation:
    APA:
    Collins, Maria C., & Moore, Joel L. Jr, & Burrows, Brian J., & Kypson, Alan P., & Muller-Borer, Barbara J.. (March 2012). Early cell loss associated with mesenchymal stem cell cardiomyoplasty. , (), - . Retrieved from http://hdl.handle.net/10342/7860

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    MLA:
    Collins, Maria C., and Moore, Joel L. Jr, and Burrows, Brian J., and Kypson, Alan P., and Muller-Borer, Barbara J.. "Early cell loss associated with mesenchymal stem cell cardiomyoplasty". . . (), March 2012. September 22, 2023. http://hdl.handle.net/10342/7860.
    Chicago:
    Collins, Maria C. and Moore, Joel L. Jr and Burrows, Brian J. and Kypson, Alan P. and Muller-Borer, Barbara J., "Early cell loss associated with mesenchymal stem cell cardiomyoplasty," , no. (March 2012), http://hdl.handle.net/10342/7860 (accessed September 22, 2023).
    AMA:
    Collins, Maria C., Moore, Joel L. Jr, Burrows, Brian J., Kypson, Alan P., Muller-Borer, Barbara J.. Early cell loss associated with mesenchymal stem cell cardiomyoplasty. . March 2012; (): . http://hdl.handle.net/10342/7860. Accessed September 22, 2023.
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