Murine mesothelin: characterization, expression, and inhibition of tumor growth in a murine model of pancreatic cancer
Author
Zervos, Emmanuel; Agle, Steven C.; Freistaedter, Andrew; Jones, Gwendolyn; Roper, Rachel
Abstract
Background: Mesothelin has attracted much interest as a tumor specific antigen; it has been reported to promote
tumor development and to be a good target for cancer treatment. Most studies to date have used human
mesothelin in immunocompromised mice. Since these models do not allow for study of the natural immune
response to mesothelin expressing tumors, we have undertaken the characterization of mouse mesothelin so the
effects of this protein can be assessed in immunocompetent mouse strains.
Methods: We analyzed mouse mesothelin expression, tissue distribution, shedding and biochemistry. In addition we constructed stable mesothelin overexpressing lines of the pancreatic cancer line Panc02 by two methods and tested them for growth and tumorigencity in vitro and in vivo.
Results: We show here that mouse mesothelin is similar to human mesothelin in biochemical characteristics, tumor expression and tissue distribution, suggesting the mouse may be a suitable model for study of mesothelin. Stable overexpression of mesothelin in a pancreatic cancer cell line did not increase cell proliferation or anchorageindependent growth in vitro, suggesting that mesothelin is not necessarily a tumor progression factor. Surprisingly overexpression of mesothelin inhibited tumor formation in vivo in immunocompetent mice.
Conclusion: The mouse may be a good model for studying mesothelin in the context of an intact immune response. Mesothelin is not necessarily a tumor progression factor, and indeed mesothelin overexpression inhibited tumor growth in immunocompetent mice.
Date
2016
Citation:
APA:
Zervos, Emmanuel, & Agle, Steven C., & Freistaedter, Andrew, & Jones, Gwendolyn, & Roper, Rachel. (January 2016).
Murine mesothelin: characterization, expression, and inhibition of tumor growth in a murine model of pancreatic cancer.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/7881
MLA:
Zervos, Emmanuel, and Agle, Steven C., and Freistaedter, Andrew, and Jones, Gwendolyn, and Roper, Rachel.
"Murine mesothelin: characterization, expression, and inhibition of tumor growth in a murine model of pancreatic cancer". .
. (),
January 2016.
April 26, 2024.
http://hdl.handle.net/10342/7881.
Chicago:
Zervos, Emmanuel and Agle, Steven C. and Freistaedter, Andrew and Jones, Gwendolyn and Roper, Rachel,
"Murine mesothelin: characterization, expression, and inhibition of tumor growth in a murine model of pancreatic cancer," , no.
(January 2016),
http://hdl.handle.net/10342/7881 (accessed
April 26, 2024).
AMA:
Zervos, Emmanuel, Agle, Steven C., Freistaedter, Andrew, Jones, Gwendolyn, Roper, Rachel.
Murine mesothelin: characterization, expression, and inhibition of tumor growth in a murine model of pancreatic cancer. .
January 2016;
():
.
http://hdl.handle.net/10342/7881. Accessed
April 26, 2024.
Collections