Claudin‑7 modulates cell‑matrix adhesion that controls cell migration, invasion and attachment of human HCC827 lung cancer cells
Author
KIM, DO HYUNG; LU, QUN; CHEN, YAN-HUA
Abstract
Claudins are a family of tight junction proteins, and serve important roles in epithelial barrier, selective ion transports and cancer metastasis. Although the exact role of claudin-7 in human lung cancer has not been completely elucidated, recent clinical studies have demonstrated that claudin-7 is associated with the survival of patients with lung cancer. Our previous studies have demonstrated that claudin-7 forms a protein complex with integrin β1 in human lung cancer cells. The knockdown (KD) of claudin-7 by short hairpin RNA (shRNA) reduced integrin β1 expression and increased the cell proliferative rate, whereas claudin-7 re-expression in the KD cells decreased the cell proliferation. It is unknown as to whether claudin-7 and integrin β1 regulate cell proliferation and invasion synergistically or independently. In the present study, it was observed that ectopic expression of integrin β1 in claudin-7 KD lung cancer cells did not reduce the cell proliferation. However, integrin β1-transfected cells migrated more effectively in wound healing and cell invasion assays and were more adhesive in a cell attachment assay when compared with those of claudin-7 KD cells. This indicates that claudin-7 controls cell proliferation, while cell attachment and motility were regulated partially through integrin β1. Additionally, claudin-7 overexpression in claudin-7 KD cells resulted in an improved ability to attach to the surface of cell culture plates and a higher expression of focal adhesion proteins when compared with claudin-7 non-KD control cells, which supports the role of claudin-7 in cell adhesion and motility. Taken together, these data suggest that claudin-7 regulates cell motility through integrin β1, providing additional insight into the roles of claudins in carcinogenesis and cancer cell metastasis.
Date
2018-12-31
Citation:
APA:
KIM, DO HYUNG, & LU, QUN, & CHEN, YAN-HUA. (December 2018).
Claudin‑7 modulates cell‑matrix adhesion that controls cell migration, invasion and attachment of human HCC827 lung cancer cells.
Oncology Letters,
(17:3), p.2890-2896. Retrieved from
http://hdl.handle.net/10342/7982
MLA:
KIM, DO HYUNG, and LU, QUN, and CHEN, YAN-HUA.
"Claudin‑7 modulates cell‑matrix adhesion that controls cell migration, invasion and attachment of human HCC827 lung cancer cells". Oncology Letters.
17:3. (2890-2896.),
December 2018.
April 19, 2021.
http://hdl.handle.net/10342/7982.
Chicago:
KIM, DO HYUNG and LU, QUN and CHEN, YAN-HUA,
"Claudin‑7 modulates cell‑matrix adhesion that controls cell migration, invasion and attachment of human HCC827 lung cancer cells," Oncology Letters 17, no.
3 (December 2018),
http://hdl.handle.net/10342/7982 (accessed
April 19, 2021).
AMA:
KIM, DO HYUNG, LU, QUN, CHEN, YAN-HUA.
Claudin‑7 modulates cell‑matrix adhesion that controls cell migration, invasion and attachment of human HCC827 lung cancer cells. Oncology Letters.
December 2018;
17(3)
2890-2896. http://hdl.handle.net/10342/7982. Accessed
April 19, 2021.
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