A single dose of trichloroethylene given during development does not substantially alter markers of neuroinflammation in brains of adult mice
Author
Meadows, Jacqueline R.; Parker, Chevonne; Gilbert, Kathleen M.; Blossom, Sarah J.; DeWitt, Jamie C.
Abstract
Trichloroethylene (TCE) is a widespread environmental contaminant associated with developmental immu- notoxicity and neurotoxicity. Previous studies have shown that MRLþ/þ mice exposed to TCE from gesta- tion through early-life demonstrate robust increases in inflammatory markers in peripheral CD4þ T-cells, as well as glutathione depletion and increased oxidative stress in cerebellum-associated with alterations in behavior. Since increased oxidative stress is associated with neuroinflammation, we hypothesized that neuroinflammatory markers could be altered relative to unexposed mice. MRLþ/þ mice were given 0.5mg/ml of TCE in vehicle or vehicle (water with 1% Alkamuls EL-620) from conception through early adulthood via drinking water to dams and then directly to post-weaning offspring. Animals were euthan- ized at 49days of age and levels of pro- and anti-inflammatory cytokines, density of T-cell staining, and micro-glial morphology were evaluated in brains to begin to ascertain a neuroinflammatory profile. Levels of IL-6 were decreased in female animals and while not statistically significant, and levels of IL-10 were higher in brains of exposed male and female animals. Supportive of this observation, although not statis- tically significant, the number of ameboid microglia was higher in exposed relative to unexposed animals. This overall profile suggests the emergence of an anti-inflammatory/neuroprotective phenotype in exposed animals, possibly as a compensatory response to neuroinflammation that is known to be induced by developmental exposure to TCE.
Date
2017-04-03
Citation:
APA:
Meadows, Jacqueline R., & Parker, Chevonne, & Gilbert, Kathleen M., & Blossom, Sarah J., & DeWitt, Jamie C.. (April 2017).
A single dose of trichloroethylene given during development does not substantially alter markers of neuroinflammation in brains of adult mice.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/8021
MLA:
Meadows, Jacqueline R., and Parker, Chevonne, and Gilbert, Kathleen M., and Blossom, Sarah J., and DeWitt, Jamie C..
"A single dose of trichloroethylene given during development does not substantially alter markers of neuroinflammation in brains of adult mice". .
. (),
April 2017.
December 11, 2023.
http://hdl.handle.net/10342/8021.
Chicago:
Meadows, Jacqueline R. and Parker, Chevonne and Gilbert, Kathleen M. and Blossom, Sarah J. and DeWitt, Jamie C.,
"A single dose of trichloroethylene given during development does not substantially alter markers of neuroinflammation in brains of adult mice," , no.
(April 2017),
http://hdl.handle.net/10342/8021 (accessed
December 11, 2023).
AMA:
Meadows, Jacqueline R., Parker, Chevonne, Gilbert, Kathleen M., Blossom, Sarah J., DeWitt, Jamie C..
A single dose of trichloroethylene given during development does not substantially alter markers of neuroinflammation in brains of adult mice. .
April 2017;
():
.
http://hdl.handle.net/10342/8021. Accessed
December 11, 2023.
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