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    Rapid development of Purkinje cell excitability, functional cerebellar circuit, and afferent sensory input to cerebellum in zebrafish

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    Author
    Hsieh, Jui-Yi; Ulrich, Brittany; Issa, Fadi A; Wan, Jijun; Papazian, Diane M
    Abstract
    The zebrafish has significant advantages for studying the morphological development of the brain. However, little is known about the functional development of the zebrafish brain. We used patch clamp electrophysiology in live animals to investigate the emergence of excitability in cerebellar Purkinje cells, functional maturation of the cerebellar circuit, and establishment of sensory input to the cerebellum. Purkinje cells are born at 3 days post-fertilization (dpf). By 4 dpf, Purkinje cells spontaneously fired action potentials in an irregular pattern. By 5 dpf, the frequency and regularity of tonic firing had increased significantly and most cells fired complex spikes in response to climbing fiber activation. Our data suggest that, as in mammals, Purkinje cells are initially innervated by multiple climbing fibers that are winnowed to a single input. To probe the development of functional sensory input to the cerebellum, we investigated the response of Purkinje cells to a visual stimulus consisting of a rapid change in light intensity. At 4 dpf, sudden darkness increased the rate of tonic firing, suggesting that afferent pathways carrying visual information are already active by this stage. By 5 dpf, visual stimuli also activated climbing fibers, increasing the frequency of complex spiking. Our results indicate that the electrical properties of zebrafish and mammalian Purkinje cells are highly conserved and suggest that the same ion channels, Nav1.6 and Kv3.3, underlie spontaneous pacemaking activity. Interestingly, functional development of the cerebellum is temporally correlated with the emergence of complex, visually-guided behaviors such as prey capture. Because of the rapid formation of an electrically-active cerebellum, optical transparency, and ease of genetic manipulation, the zebrafish has great potential for functionally mapping cerebellar afferent and efferent pathways and for investigating cerebellar control of motor behavior.
    URI
    http://hdl.handle.net/10342/8046
    Subject
    Purkinje cell, patch clamp, cerebellum, zebrafish, parallel fiber, climbing fiber, visual input
    Date
    2014-12-19
    Citation:
    APA:
    Hsieh, Jui-Yi, & Ulrich, Brittany, & Issa, Fadi A, & Wan, Jijun, & Papazian, Diane M. (December 2014). Rapid development of Purkinje cell excitability, functional cerebellar circuit, and afferent sensory input to cerebellum in zebrafish. Frontiers in Neural Circuits, (8:147), p.1-12. Retrieved from http://hdl.handle.net/10342/8046

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Hsieh, Jui-Yi, and Ulrich, Brittany, and Issa, Fadi A, and Wan, Jijun, and Papazian, Diane M. "Rapid development of Purkinje cell excitability, functional cerebellar circuit, and afferent sensory input to cerebellum in zebrafish". Frontiers in Neural Circuits. 8:147. (1-12.), December 2014. February 24, 2021. http://hdl.handle.net/10342/8046.
    Chicago:
    Hsieh, Jui-Yi and Ulrich, Brittany and Issa, Fadi A and Wan, Jijun and Papazian, Diane M, "Rapid development of Purkinje cell excitability, functional cerebellar circuit, and afferent sensory input to cerebellum in zebrafish," Frontiers in Neural Circuits 8, no. 147 (December 2014), http://hdl.handle.net/10342/8046 (accessed February 24, 2021).
    AMA:
    Hsieh, Jui-Yi, Ulrich, Brittany, Issa, Fadi A, Wan, Jijun, Papazian, Diane M. Rapid development of Purkinje cell excitability, functional cerebellar circuit, and afferent sensory input to cerebellum in zebrafish. Frontiers in Neural Circuits. December 2014; 8(147) 1-12. http://hdl.handle.net/10342/8046. Accessed February 24, 2021.
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