Frequency of Tongue Cleaning Impacts the Human Tongue Microbiome Composition and Enterosalivary Circulation of Nitrate
Author
Tribble, Gena D.; Angelov, Nikola; Weltman, Robin; Wang, Bing-Yan; Eswaran, Sridhar V.; Gay, Isabel C.; Parthasarathy, Kavitha; Dao, Doan-Hieu V.; Richardson, Katherine N.; Ismail, Nadia M.; Sharina, Iraida G.; Hyde, Embriette R.; Ajami, Nadim J.; Petrosino, Joseph F.; Bryan, Nathan S.
Abstract
The oral microbiome has the potential to provide an important symbiotic function in
human blood pressure physiology by contributing to the generation of nitric oxide
(NO), an essential cardiovascular signaling molecule. NO is produced by the human
body via conversion of arginine to NO by endogenous nitric oxide synthase (eNOS)
but eNOS activity varies by subject. Oral microbial communities are proposed to
supplement host NO production by reducing dietary nitrate to nitrite via bacterial
nitrate reductases. Unreduced dietary nitrate is delivered to the oral cavity in saliva, a
physiological process termed the enterosalivary circulation of nitrate. Previous studies
demonstrated that disruption of enterosalivary circulation via use of oral antiseptics
resulted in increases in systolic blood pressure. These previous studies did not
include detailed information on the oral health of enrolled subjects. Using 16S rRNA
gene sequencing and analysis, we determined whether introduction of chlorhexidine
antiseptic mouthwash for 1 week was associated with changes in tongue bacterial
communities and resting systolic blood pressure in healthy normotensive individuals
with documented oral hygiene behaviors and free of oral disease. Tongue cleaning
frequency was a predictor of chlorhexidine-induced changes in systolic blood pressure
and tongue microbiome composition. Twice-daily chlorhexidine usage was associated
with a significant increase in systolic blood pressure after 1 week of use and recovery
from use resulted in an enrichment in nitrate-reducing bacteria on the tongue. Individuals
with relatively high levels of bacterial nitrite reductases had lower resting systolic blood
pressure. These results further support the concept of a symbiotic oral microbiome
contributing to human health via the enterosalivary nitrate-nitrite-NO pathway. These data
suggest that management of the tongue microbiome by regular cleaning together with
adequate dietary intake of nitrate provide an opportunity for the improvement of resting
systolic blood pressure.
Date
2019-03-01
Citation:
APA:
Tribble, Gena D., & Angelov, Nikola, & Weltman, Robin, & Wang, Bing-Yan, & Eswaran, Sridhar V., & Gay, Isabel C., & Parthasarathy, Kavitha, & Dao, Doan-Hieu V., & Richardson, Katherine N., & Ismail, Nadia M., & Sharina, Iraida G., & Hyde, Embriette R., & Ajami, Nadim J., & Petrosino, Joseph F., & Bryan, Nathan S.. (March 2019).
Frequency of Tongue Cleaning Impacts the Human Tongue Microbiome Composition and Enterosalivary Circulation of Nitrate.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/8084
MLA:
Tribble, Gena D., and Angelov, Nikola, and Weltman, Robin, and Wang, Bing-Yan, and Eswaran, Sridhar V., and Gay, Isabel C., and Parthasarathy, Kavitha, and Dao, Doan-Hieu V., and Richardson, Katherine N., and Ismail, Nadia M., and Sharina, Iraida G., and Hyde, Embriette R., and Ajami, Nadim J., and Petrosino, Joseph F., and Bryan, Nathan S..
"Frequency of Tongue Cleaning Impacts the Human Tongue Microbiome Composition and Enterosalivary Circulation of Nitrate". .
. (),
March 2019.
September 22, 2023.
http://hdl.handle.net/10342/8084.
Chicago:
Tribble, Gena D. and Angelov, Nikola and Weltman, Robin and Wang, Bing-Yan and Eswaran, Sridhar V. and Gay, Isabel C. and Parthasarathy, Kavitha and Dao, Doan-Hieu V. and Richardson, Katherine N. and Ismail, Nadia M. and Sharina, Iraida G. and Hyde, Embriette R. and Ajami, Nadim J. and Petrosino, Joseph F. and Bryan, Nathan S.,
"Frequency of Tongue Cleaning Impacts the Human Tongue Microbiome Composition and Enterosalivary Circulation of Nitrate," , no.
(March 2019),
http://hdl.handle.net/10342/8084 (accessed
September 22, 2023).
AMA:
Tribble, Gena D., Angelov, Nikola, Weltman, Robin, Wang, Bing-Yan, Eswaran, Sridhar V., Gay, Isabel C., Parthasarathy, Kavitha, Dao, Doan-Hieu V., Richardson, Katherine N., Ismail, Nadia M., Sharina, Iraida G., Hyde, Embriette R., Ajami, Nadim J., Petrosino, Joseph F., Bryan, Nathan S..
Frequency of Tongue Cleaning Impacts the Human Tongue Microbiome Composition and Enterosalivary Circulation of Nitrate. .
March 2019;
():
.
http://hdl.handle.net/10342/8084. Accessed
September 22, 2023.
Collections