Insulin/IGF1-PI3K-dependent nucleolar localization of a glycolytic enzyme – phosphoglycerate mutase 2, is necessary for proper structure of nucleolus and RNA synthesis
Author
Gizak, Agnieszka; Grenda, Marcin; Mamczur, Piotr; Wisniewski, Janusz; Sucharsk, Filip; Silberring, Jerzy; McCubrey, James A.; Wisniewski, Jacek R.; Rakus, Dariusz
Abstract
Phosphoglycerate mutase (PGAM), a conserved, glycolytic enzyme has been
found in nucleoli of cancer cells. Here, we present evidence that accumulation of
PGAM in the nucleolus is a universal phenomenon concerning not only neoplastically
transformed but also non-malignant cells. Nucleolar localization of the enzyme is
dependent on the presence of the PGAM2 (muscle) subunit and is regulated by insulin/
IGF-1–PI3K signaling pathway as well as drugs influencing ribosomal biogenesis. We
document that PGAM interacts with several 40S and 60S ribosomal proteins and that
silencing of PGAM2 expression results in disturbance of nucleolar structure, inhibition
of RNA synthesis and decrease of the mitotic index of squamous cell carcinoma cells.
We conclude that presence of PGAM in the nucleolus is a prerequisite for synthesis
and initial assembly of new pre-ribosome subunits.
Date
2015
Citation:
APA:
Gizak, Agnieszka, & Grenda, Marcin, & Mamczur, Piotr, & Wisniewski, Janusz, & Sucharsk, Filip, & Silberring, Jerzy, & McCubrey, James A., & Wisniewski, Jacek R., & Rakus, Dariusz. (January 2015).
Insulin/IGF1-PI3K-dependent nucleolar localization of a glycolytic enzyme – phosphoglycerate mutase 2, is necessary for proper structure of nucleolus and RNA synthesis.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/8085
MLA:
Gizak, Agnieszka, and Grenda, Marcin, and Mamczur, Piotr, and Wisniewski, Janusz, and Sucharsk, Filip, and Silberring, Jerzy, and McCubrey, James A., and Wisniewski, Jacek R., and Rakus, Dariusz.
"Insulin/IGF1-PI3K-dependent nucleolar localization of a glycolytic enzyme – phosphoglycerate mutase 2, is necessary for proper structure of nucleolus and RNA synthesis". .
. (),
January 2015.
June 29, 2024.
http://hdl.handle.net/10342/8085.
Chicago:
Gizak, Agnieszka and Grenda, Marcin and Mamczur, Piotr and Wisniewski, Janusz and Sucharsk, Filip and Silberring, Jerzy and McCubrey, James A. and Wisniewski, Jacek R. and Rakus, Dariusz,
"Insulin/IGF1-PI3K-dependent nucleolar localization of a glycolytic enzyme – phosphoglycerate mutase 2, is necessary for proper structure of nucleolus and RNA synthesis," , no.
(January 2015),
http://hdl.handle.net/10342/8085 (accessed
June 29, 2024).
AMA:
Gizak, Agnieszka, Grenda, Marcin, Mamczur, Piotr, Wisniewski, Janusz, Sucharsk, Filip, Silberring, Jerzy, McCubrey, James A., Wisniewski, Jacek R., Rakus, Dariusz.
Insulin/IGF1-PI3K-dependent nucleolar localization of a glycolytic enzyme – phosphoglycerate mutase 2, is necessary for proper structure of nucleolus and RNA synthesis. .
January 2015;
():
.
http://hdl.handle.net/10342/8085. Accessed
June 29, 2024.
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