Author | Gizak, Agnieszka | |
Author | Grenda, Marcin | |
Author | Mamczur, Piotr | |
Author | Wisniewski, Janusz | |
Author | Sucharsk, Filip | |
Author | Silberring, Jerzy | |
Author | McCubrey, James A. | |
Author | Wisniewski, Jacek R. | |
Author | Rakus, Dariusz | |
Date Accessioned | 2020-04-13T17:07:28Z | |
Date Available | 2020-04-13T17:07:28Z | |
Date of Issue | 2015 | |
Identifier (URI) | http://hdl.handle.net/10342/8085 | |
Description | Phosphoglycerate mutase (PGAM), a conserved, glycolytic enzyme has been
found in nucleoli of cancer cells. Here, we present evidence that accumulation of
PGAM in the nucleolus is a universal phenomenon concerning not only neoplastically
transformed but also non-malignant cells. Nucleolar localization of the enzyme is
dependent on the presence of the PGAM2 (muscle) subunit and is regulated by insulin/
IGF-1–PI3K signaling pathway as well as drugs influencing ribosomal biogenesis. We
document that PGAM interacts with several 40S and 60S ribosomal proteins and that
silencing of PGAM2 expression results in disturbance of nucleolar structure, inhibition
of RNA synthesis and decrease of the mitotic index of squamous cell carcinoma cells.
We conclude that presence of PGAM in the nucleolus is a prerequisite for synthesis
and initial assembly of new pre-ribosome subunits. | en_US |
Subject | squamous cell carcinoma, PGAM2, rRNA, ribosome assembly, multifunctional enzyme | en_US |
Title | Insulin/IGF1-PI3K-dependent nucleolar localization of a glycolytic enzyme – phosphoglycerate mutase 2, is necessary for proper structure of nucleolus and RNA synthesis | en_US |
Type | Article | en_US |
Identifier (DOI) | 10.18632/oncotarget.4044 | |
Journal Name | Oncotarget | en_US |
Journal Volume | 6 | en_US |
Journal Issue | 19 | en_US |