Global redox proteome and phosphoproteome analysis reveals redox switch in Akt
Author
Su, Zhiduan; Burchfield, James G.; Yang, Pengyi; Humphrey, Sean J.; Yang, Guang; Francis, Deanne; Yasmin, Sabina; Shin, Sung-Young; Norris, Dougall M.; Kearney, Alison L.; Astore, Miro A.; Scavuzzo, Jonathan; Fisher-Wellman, Kelsey H.; Wang, Qiao-Ping; Parker, Benjamin L.; Neely, G. Gregory; Vafaee, Fatemeh; Chiu, Joyce; Yeo, Reichelle; Hogg, Philip J.; Fazakerley, Daniel J.; Nguyen, Lan K.; Kuyucak, Serdar; James, David E.
Abstract
Protein oxidation sits at the intersection of multiple signalling pathways, yet the magnitude and extent of crosstalk between oxidation and other post-translational modifications remains unclear. Here, we delineate global changes in adipocyte signalling networks following acute oxidative stress and reveal considerable crosstalk between cysteine oxidation and phosphorylation-based signalling. Oxidation of key regulatory kinases, including Akt, mTOR and AMPK influences the fidelity rather than their absolute activation state, highlighting an unappreciated interplay between these modifications. Mechanistic analysis of the redox regulation of Akt identified two cysteine residues in the pleckstrin homology domain (C60 and C77) to be reversibly oxidized. Oxidation at these sites affected Akt recruitment to the plasma membrane by stabilizing the PIP3 binding pocket. Our data provide insights into the interplay between oxidative stress-derived redox signalling and protein phosphorylation networks and serve as a resource for understanding the contribution of cellular oxidation to a range of diseases.
Date
2019-12-02
Citation:
APA:
Su, Zhiduan, & Burchfield, James G., & Yang, Pengyi, & Humphrey, Sean J., & Yang, Guang, & Francis, Deanne, & Yasmin, Sabina, & Shin, Sung-Young, & Norris, Dougall M., & Kearney, Alison L., & Astore, Miro A., & Scavuzzo, Jonathan, & Fisher-Wellman, Kelsey H., & Wang, Qiao-Ping, & Parker, Benjamin L., & Neely, G. Gregory, & Vafaee, Fatemeh, & Chiu, Joyce, & Yeo, Reichelle, & Hogg, Philip J., & Fazakerley, Daniel J., & Nguyen, Lan K., & Kuyucak, Serdar, & James, David E.. (December 2019).
Global redox proteome and phosphoproteome analysis reveals redox switch in Akt.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/8087
MLA:
Su, Zhiduan, and Burchfield, James G., and Yang, Pengyi, and Humphrey, Sean J., and Yang, Guang, and Francis, Deanne, and Yasmin, Sabina, and Shin, Sung-Young, and Norris, Dougall M., and Kearney, Alison L., and Astore, Miro A., and Scavuzzo, Jonathan, and Fisher-Wellman, Kelsey H., and Wang, Qiao-Ping, and Parker, Benjamin L., and Neely, G. Gregory, and Vafaee, Fatemeh, and Chiu, Joyce, and Yeo, Reichelle, and Hogg, Philip J., and Fazakerley, Daniel J., and Nguyen, Lan K., and Kuyucak, Serdar, and James, David E..
"Global redox proteome and phosphoproteome analysis reveals redox switch in Akt". .
. (),
December 2019.
September 30, 2023.
http://hdl.handle.net/10342/8087.
Chicago:
Su, Zhiduan and Burchfield, James G. and Yang, Pengyi and Humphrey, Sean J. and Yang, Guang and Francis, Deanne and Yasmin, Sabina and Shin, Sung-Young and Norris, Dougall M. and Kearney, Alison L. and Astore, Miro A. and Scavuzzo, Jonathan and Fisher-Wellman, Kelsey H. and Wang, Qiao-Ping and Parker, Benjamin L. and Neely, G. Gregory and Vafaee, Fatemeh and Chiu, Joyce and Yeo, Reichelle and Hogg, Philip J. and Fazakerley, Daniel J. and Nguyen, Lan K. and Kuyucak, Serdar and James, David E.,
"Global redox proteome and phosphoproteome analysis reveals redox switch in Akt," , no.
(December 2019),
http://hdl.handle.net/10342/8087 (accessed
September 30, 2023).
AMA:
Su, Zhiduan, Burchfield, James G., Yang, Pengyi, Humphrey, Sean J., Yang, Guang, Francis, Deanne, Yasmin, Sabina, Shin, Sung-Young, Norris, Dougall M., Kearney, Alison L., Astore, Miro A., Scavuzzo, Jonathan, Fisher-Wellman, Kelsey H., Wang, Qiao-Ping, Parker, Benjamin L., Neely, G. Gregory, Vafaee, Fatemeh, Chiu, Joyce, Yeo, Reichelle, Hogg, Philip J., Fazakerley, Daniel J., Nguyen, Lan K., Kuyucak, Serdar, James, David E..
Global redox proteome and phosphoproteome analysis reveals redox switch in Akt. .
December 2019;
():
.
http://hdl.handle.net/10342/8087. Accessed
September 30, 2023.
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