De Novo Transcriptome Assembly and Comparative Analysis Elucidate Complicated Mechanism Regulating Astragalus chrysochlorus Response to Selenium Stimuli
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Date
2015
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Authors
Çakır, Özgür
Turgut-Kara1, Neslihan
Arı, Şule
Zhang, Baohong
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Abstract
Astragalus species are medicinal plants that are used in the world for years. Some Astragalus species are known for selenium accumulation and tolerance and one of them is
Astragalus chrysochlorus, a secondary selenium accumulator. In this study, we employed
Illumina deep sequencing technology for the first time to de novo assemble A. chrysochlorus transcriptome and identify the differentially expressed genes after selenate treatment. Totally, 59,656 unigenes were annotated with different databases and 53,960
unigenes were detected in NR database. Transcriptome in A. chrysochlorus is closer to
Glycine max than other plant species with 43,1 percentage of similarity. Annotated unigenes were also used for gene ontology enrichment and pathway enrichment analysis.
The most significant genes and pathways were ABC transporters, plant pathogen interaction, biosynthesis of secondary metabolites and carbohydrate metabolism. Our results will
help to enlighten the selenium accumulation and tolerance mechanisms, respectively in
plants
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DOI
10.1371/journal.pone.0135677