Long Noncoding RNA Expression Signatures of Metastatic Nasopharyngeal Carcinoma and Their Prognostic Value
Zhang, Wei; Wang, Lin; Zheng, Fang; Zou, Ruhai; Xie, Changging; Guo, Qiannan; Hu, Qian; Chen, Jianing; Yang, Xing; Yao, Herui; Song, Erwei; Xiang, Yangun
Long noncoding RNAs (lncRNAs) have recently been found to play important roles in various cancer types. The elucidation of genome-wide lncRNA expression patterns in metastatic nasopharyngeal carcinoma (NPC) could reveal novel mechanisms underlying NPC carcinogenesis and progression. In this study, lncRNA expression profiling was performed on metastatic and primary NPC tumors, and the differentially expressed lncRNAs between these samples were identified. A total of 33,045 lncRNA probes were generated for our microarray based on authoritative data sources, including RefSeq, UCSC Knowngenes, Ensembl, and related literature. Using these probes, 8,088 lncRNAs were found to be significantly differentially expressed (≥2-fold). To identify the prognostic value of these differentially expressed lncRNAs, four lncRNAs (LOC84740, ENST00000498296, AL359062, and ENST00000438550) were selected; their expression levels were measured in an independent panel of 106 primary NPC samples via QPCR. Among these lncRNAs, ENST00000438550 expression was demonstrated to be significantly correlated with NPC disease progression. A survival analysis showed that a high expression level of ENST00000438550 was an independent indicator of disease progression in NPC patients (P = 0.01). In summary, this study may provide novel diagnostic and prognostic biomarkers for NPC, as well as a novel understanding of the mechanism underlying NPC metastasis and potential targets for future treatment.
Zhang, Wei, & Wang, Lin, & Zheng, Fang, & Zou, Ruhai, & Xie, Changging, & Guo, Qiannan, & Hu, Qian, & Chen, Jianing, & Yang, Xing, & Yao, Herui, & Song, Erwei, & Xiang, Yangun. (January 2015). Long Noncoding RNA Expression Signatures of Metastatic Nasopharyngeal Carcinoma and Their Prognostic Value. BioMed research international, (2015:2015), p.. Retrieved from http://hdl.handle.net/10342/8155
Zhang, Wei, and Wang, Lin, and Zheng, Fang, and Zou, Ruhai, and Xie, Changging, and Guo, Qiannan, and Hu, Qian, and Chen, Jianing, and Yang, Xing, and Yao, Herui, and Song, Erwei, and Xiang, Yangun. "Long Noncoding RNA Expression Signatures of Metastatic Nasopharyngeal Carcinoma and Their Prognostic Value". BioMed research international. 2015:2015. (.), January 2015. September 23, 2020. http://hdl.handle.net/10342/8155.
Zhang, Wei and Wang, Lin and Zheng, Fang and Zou, Ruhai and Xie, Changging and Guo, Qiannan and Hu, Qian and Chen, Jianing and Yang, Xing and Yao, Herui and Song, Erwei and Xiang, Yangun, "Long Noncoding RNA Expression Signatures of Metastatic Nasopharyngeal Carcinoma and Their Prognostic Value," BioMed research international 2015, no. 2015 (January 2015), http://hdl.handle.net/10342/8155 (accessed September 23, 2020).
Zhang, Wei, Wang, Lin, Zheng, Fang, Zou, Ruhai, Xie, Changging, Guo, Qiannan, Hu, Qian, Chen, Jianing, Yang, Xing, Yao, Herui, Song, Erwei, Xiang, Yangun. Long Noncoding RNA Expression Signatures of Metastatic Nasopharyngeal Carcinoma and Their Prognostic Value. BioMed research international. January 2015; 2015(2015) . http://hdl.handle.net/10342/8155. Accessed September 23, 2020.