• Find People
  • Campus Map
  • PiratePort
  • A-Z
    • About
    • Submit
    • Browse
    • Login
    View Item 
    •   ScholarShip Home
    • Other Campus Research
    • Open Access
    • View Item
    •   ScholarShip Home
    • Other Campus Research
    • Open Access
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of The ScholarShipCommunities & CollectionsDateAuthorsTitlesSubjectsTypeDate SubmittedThis CollectionDateAuthorsTitlesSubjectsTypeDate Submitted

    My Account

    Login

    Statistics

    View Google Analytics Statistics

    EphrinA1-Fc attenuates myocardial ischemia/ reperfusion injury in mice

    Thumbnail
    View/ Open
    journal.pone.0189307.pdf (11.12Mb)

    Show full item record
    Author
    DuSablon, Augustin; Parks, Justin; Whitehurst, K’Shylah; Estes, H. Lynn; Chase, Robert; Vlahos, Eleftherios; Sharma, Uma; Wert, David; Virag, Jitka
    Abstract
    EphrinA1, a membrane-bound receptor tyrosine kinase ligand expressed in healthy car- diomyocytes, is lost in injured cells following myocardial infarction. Previously, we have reported that a single intramyocardial injection of chimeric ephrinA1-Fc at the time of ische- mia reduced injury in the nonreperfused myocardium by 50% at 4 days post-MI by reducing apoptosis and inflammatory cell infiltration. In a clinically relevant model of acute ischemia (30min)/reperfusion (24hr or 4 days) injury, we now demonstrate that ephrinA1-Fc reduces infarct size by 46% and completely preserves cardiac function (ejection fraction, fractional shortening, and chamber dimensions) in the short-term (24hrs post-MI) as well as long-term (4 days). At 24 hours post-MI, diminished serum inflammatory cell chemoattractants in ephrinA1-Fc-treated mice reduces recruitment of neutrophils and leukocytes into the myo- cardium. Differences in relative expression levels of EphA-Rs are described in the context of their putative role in mediating cardioprotection. Validation by Western blotting of selected targets from mass spectrometry analyses of pooled samples of left ventricular tissue ho- mogenates from mice that underwent 30min ischemia and 24hr of reperfusion (I/R) indicates that ephrinA1-Fc administration alters several regulators of signaling pathways that attenu- ate apoptosis, promote autophagy, and shift from FA metabolism in favor of increased gly- colysis to optimize anaerobic ATP production. Taken together, reduced injury is due a combination of adaptive metabolic reprogramming, improved cell survival, and decreased inflammatory cell recruitment, suggesting that ephrinA1-Fc enhances the capacity of the heart to withstand an ischemic insult.
    URI
    http://hdl.handle.net/10342/8179
    Date
    2017-12-13
    Citation:
    APA:
    DuSablon, Augustin, & Parks, Justin, & Whitehurst, K’Shylah, & Estes, H. Lynn, & Chase, Robert, & Vlahos, Eleftherios, & Sharma, Uma, & Wert, David, & Virag, Jitka. (December 2017). EphrinA1-Fc attenuates myocardial ischemia/ reperfusion injury in mice. , (), - . Retrieved from http://hdl.handle.net/10342/8179

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    DuSablon, Augustin, and Parks, Justin, and Whitehurst, K’Shylah, and Estes, H. Lynn, and Chase, Robert, and Vlahos, Eleftherios, and Sharma, Uma, and Wert, David, and Virag, Jitka. "EphrinA1-Fc attenuates myocardial ischemia/ reperfusion injury in mice". . . (), December 2017. November 30, 2023. http://hdl.handle.net/10342/8179.
    Chicago:
    DuSablon, Augustin and Parks, Justin and Whitehurst, K’Shylah and Estes, H. Lynn and Chase, Robert and Vlahos, Eleftherios and Sharma, Uma and Wert, David and Virag, Jitka, "EphrinA1-Fc attenuates myocardial ischemia/ reperfusion injury in mice," , no. (December 2017), http://hdl.handle.net/10342/8179 (accessed November 30, 2023).
    AMA:
    DuSablon, Augustin, Parks, Justin, Whitehurst, K’Shylah, Estes, H. Lynn, Chase, Robert, Vlahos, Eleftherios, Sharma, Uma, Wert, David, Virag, Jitka. EphrinA1-Fc attenuates myocardial ischemia/ reperfusion injury in mice. . December 2017; (): . http://hdl.handle.net/10342/8179. Accessed November 30, 2023.
    Collections
    • Open Access

    xmlui.ArtifactBrowser.ItemViewer.elsevier_entitlement

    East Carolina University has created ScholarShip, a digital archive for the scholarly output of the ECU community.

    • About
    • Contact Us
    • Send Feedback