Hypoxia and Hypoglycemia synergistically regulate mRNA stability
Author
Carraway, Kristen R.; Johnson, Ellen M.; Kauffmann, Travis C.; Fry, Nate J.; Mansfield, Kyle D.
Abstract
Ischemic events, common in many diseases, result from decreased blood flow and impaired delivery of oxygen and glucose to tissues of the body. While much is known about the cellular transcriptional response to ischemia, much less is known about the posttranscriptional response to oxygen and glucose deprivation. The goal of this project was to investigate one such posttranscriptional response, the regulation of mRNA stability. To that end, we have identified several novel ischemia-related mRNAs that are synergistically stabilized by oxygen and glucose deprivation including VEGF, MYC, MDM2, and CYR61. This increase in mRNA half-life requires the synergistic effects of both low oxygen (1%) as well as low glucose ( 1 g/L) conditions. Oxygen or glucose deprivation alone fails to initiate the response, as exposure to either high glucose (4 g/L) or normoxic conditions inhibits the response. Furthermore, in response to hypoxia/hypoglycemia, the identified mRNAs are released from the RNA binding protein KHSRP which likely contributes to their stabilization.
Date
2017-07
Citation:
APA:
Carraway, Kristen R., & Johnson, Ellen M., & Kauffmann, Travis C., & Fry, Nate J., & Mansfield, Kyle D.. (July 2017).
Hypoxia and Hypoglycemia synergistically regulate mRNA stability.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/8191
MLA:
Carraway, Kristen R., and Johnson, Ellen M., and Kauffmann, Travis C., and Fry, Nate J., and Mansfield, Kyle D..
"Hypoxia and Hypoglycemia synergistically regulate mRNA stability". .
. (),
July 2017.
May 10, 2024.
http://hdl.handle.net/10342/8191.
Chicago:
Carraway, Kristen R. and Johnson, Ellen M. and Kauffmann, Travis C. and Fry, Nate J. and Mansfield, Kyle D.,
"Hypoxia and Hypoglycemia synergistically regulate mRNA stability," , no.
(July 2017),
http://hdl.handle.net/10342/8191 (accessed
May 10, 2024).
AMA:
Carraway, Kristen R., Johnson, Ellen M., Kauffmann, Travis C., Fry, Nate J., Mansfield, Kyle D..
Hypoxia and Hypoglycemia synergistically regulate mRNA stability. .
July 2017;
():
.
http://hdl.handle.net/10342/8191. Accessed
May 10, 2024.
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