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    Hypoxia inducible factor-1α-dependent epithelial to mesenchymal transition under hypoxic conditions in prostate cancer cells

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    Author
    Li, Mingchuan; Wang, Yong Xing; Luo, Yong; Zhao, Jiahui; Li, Qing; Zhang, Jiao; Jiang, Yongguang
    Abstract
    Prostate cancer is the most commonly diagnosed cancer in men and the second leading cause of cancer death. Hypoxia is an environmental stimulus that plays an important role in the development and cancer progression especially for solid tumors. The key regulator under hypoxic conditions is stabilized hypoxia-inducible factor (HIF)-1α. In the present study, immune-fluorescent staining, siRNAs, qRT-PC, immunoblotting, cell migration and invasion assays were carried out to test typical epithelial to mesenchymal transition under hypoxia and the key regulators of this process in PC3, a human prostate cancer cell line. Our data demonstrated that hypoxia induces diverse molecular, phenotypic and functional changes in prostate cancer cells that are consistent with EMT. We also showed that a cell signal factor such as HIF-1α, which might be stabilized under hypoxic environment, is involved in EMT and cancer cell invasive potency. The induced hypoxia could be blocked by HIF-1α gene silencing and reoxygenation of EMT in prostate cancer cells, hypoxia partially reversed accompanied by a process of mesenchymal-epithelial reverting transition (MErT). EMT might be induced by activation of HIF-1α-dependent cell signaling in hypoxic prostate cancer cells.
    URI
    http://hdl.handle.net/10342/8200
    Date
    2016
    Citation:
    APA:
    Li, Mingchuan, & Wang, Yong Xing, & Luo, Yong, & Zhao, Jiahui, & Li, Qing, & Zhang, Jiao, & Jiang, Yongguang. (January 2016). Hypoxia inducible factor-1α-dependent epithelial to mesenchymal transition under hypoxic conditions in prostate cancer cells. , (), - . Retrieved from http://hdl.handle.net/10342/8200

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Li, Mingchuan, and Wang, Yong Xing, and Luo, Yong, and Zhao, Jiahui, and Li, Qing, and Zhang, Jiao, and Jiang, Yongguang. "Hypoxia inducible factor-1α-dependent epithelial to mesenchymal transition under hypoxic conditions in prostate cancer cells". . . (), January 2016. December 11, 2023. http://hdl.handle.net/10342/8200.
    Chicago:
    Li, Mingchuan and Wang, Yong Xing and Luo, Yong and Zhao, Jiahui and Li, Qing and Zhang, Jiao and Jiang, Yongguang, "Hypoxia inducible factor-1α-dependent epithelial to mesenchymal transition under hypoxic conditions in prostate cancer cells," , no. (January 2016), http://hdl.handle.net/10342/8200 (accessed December 11, 2023).
    AMA:
    Li, Mingchuan, Wang, Yong Xing, Luo, Yong, Zhao, Jiahui, Li, Qing, Zhang, Jiao, Jiang, Yongguang. Hypoxia inducible factor-1α-dependent epithelial to mesenchymal transition under hypoxic conditions in prostate cancer cells. . January 2016; (): . http://hdl.handle.net/10342/8200. Accessed December 11, 2023.
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