WormGender – Open-Source Software for Automatic Caenorhabditis elegans Sex Ratio Measurement
Author
Labocha, Marta K.; Jung, Sang-Kyu; Aleman-Meza, Boanerges; Liu, Zheng; Zhong, Weiwei
Abstract
Fast and quantitative analysis of animal phenotypes is one of the major challenges of current biology. Here we report the WormGender open-source software, which is designed for
accurate quantification of sex ratio in Caenorhabditis elegans. The software functions
include, i) automatic recognition and counting of adult hermaphrodites and males, ii) a manual inspection feature that enables manual correction of errors, and iii) flexibility to use new
training images to optimize the software for different imaging conditions. We evaluated the
performance of our software by comparing manual and automated assessment of sex ratio.
Our data showed that the WormGender software provided overall accurate sex ratio measurements. We further demonstrated the usage of WormGender by quantifying the high
incidence of male (him) phenotype in 27 mutant strains. Mutants of nine genes (brc-1,
C30G12.6, cep-1, coh-3, him-3, him-5, him-8, skr-1, unc-86) showed significant him phenotype. The WormGender is written in Java and can be installed and run on both Windows
and Mac platforms. The source code is freely available together with a user manual and
sample data at http://www.QuantWorm.org/. The source code and sample data are also
available at http://dx.doi.org/10.6084/m9.figshare.1541248.
Date
2015
Citation:
APA:
Labocha, Marta K., & Jung, Sang-Kyu, & Aleman-Meza, Boanerges, & Liu, Zheng, & Zhong, Weiwei. (January 2015).
WormGender – Open-Source Software for Automatic Caenorhabditis elegans Sex Ratio Measurement.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/8205
MLA:
Labocha, Marta K., and Jung, Sang-Kyu, and Aleman-Meza, Boanerges, and Liu, Zheng, and Zhong, Weiwei.
"WormGender – Open-Source Software for Automatic Caenorhabditis elegans Sex Ratio Measurement". .
. (),
January 2015.
September 27, 2023.
http://hdl.handle.net/10342/8205.
Chicago:
Labocha, Marta K. and Jung, Sang-Kyu and Aleman-Meza, Boanerges and Liu, Zheng and Zhong, Weiwei,
"WormGender – Open-Source Software for Automatic Caenorhabditis elegans Sex Ratio Measurement," , no.
(January 2015),
http://hdl.handle.net/10342/8205 (accessed
September 27, 2023).
AMA:
Labocha, Marta K., Jung, Sang-Kyu, Aleman-Meza, Boanerges, Liu, Zheng, Zhong, Weiwei.
WormGender – Open-Source Software for Automatic Caenorhabditis elegans Sex Ratio Measurement. .
January 2015;
():
.
http://hdl.handle.net/10342/8205. Accessed
September 27, 2023.
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