Analysis of the B-RAFV600E mutation in cutaneous melanoma patients with occupational sun exposure
Candido, Saverio; Rapisarda, Venerando; Marconi, Andrea; Malaponte, Grazia; Bevelacqua, Valentina; Gangemi, Pietro; Scalisi, Aurora; McCubrey, James A.; Maestro, Roberta; Spandidos, Demetrios A.; Fenga, Concettina; Libra, Massimo
Sun-exposure is one of the risk factors associated with the development of a cutaneous neoplasm. In melanoma, the Ras-Raf-MEK-ERK (MAPK) signaling pathway is constitutively activated through multiple mechanisms, including B-RAF mutation. It has been hypothesized that B-RAF mutations in melanocytic lesions arise from DNA damage induced by ultraviolet (UV) radiation. However, it is still discussed if B-RAF mutations are associated with melanoma patients exposed to the sun. Therefore, in the present study, the known B-RAFV600E mutation was analysed in melanoma samples from 30 indoor and 38 outdoor workers. B-RAFV600E mutation was detected in 52 and 73% of outdoor workers and indoor workers, respectively. Of note, this mutation was identified in 12 of 14 (85%) melanoma of the trunk diagnosed in indoor workers and in 9 of 19 (47%) samples from outdoor workers (p=0.03). By analyzing melanomas of other body sites, no statistical difference in the frequency of B-RAFV600E mutation was identified between the groups of workers. It appears that the mutation detected among indoor workers may be associated with a recreational or intermittent exposure to the sun, as usually the trunk is a sun-protected body site. Overall, these data indicate that the B-RAFV600E mutation detected in melanoma is not associated with a chronic exposure to the sun. Mutations detected in other genes may also contribute to melanoma development in the subset of patients exposed to UV radiation.
Candido, Saverio, & Rapisarda, Venerando, & Marconi, Andrea, & Malaponte, Grazia, & Bevelacqua, Valentina, & Gangemi, Pietro, & Scalisi, Aurora, & McCubrey, James A., & Maestro, Roberta, & Spandidos, Demetrios A., & Fenga, Concettina, & Libra, Massimo. (January 2014). Analysis of the B-RAFV600E mutation in cutaneous melanoma patients with occupational sun exposure. Oncology Reports; Athens, (31:3), p.1079-1082. Retrieved from http://hdl.handle.net/10342/8210
Candido, Saverio, and Rapisarda, Venerando, and Marconi, Andrea, and Malaponte, Grazia, and Bevelacqua, Valentina, and Gangemi, Pietro, and Scalisi, Aurora, and McCubrey, James A., and Maestro, Roberta, and Spandidos, Demetrios A., and Fenga, Concettina, and Libra, Massimo. "Analysis of the B-RAFV600E mutation in cutaneous melanoma patients with occupational sun exposure". Oncology Reports; Athens. 31:3. (1079-1082.), January 2014. December 05, 2020. http://hdl.handle.net/10342/8210.
Candido, Saverio and Rapisarda, Venerando and Marconi, Andrea and Malaponte, Grazia and Bevelacqua, Valentina and Gangemi, Pietro and Scalisi, Aurora and McCubrey, James A. and Maestro, Roberta and Spandidos, Demetrios A. and Fenga, Concettina and Libra, Massimo, "Analysis of the B-RAFV600E mutation in cutaneous melanoma patients with occupational sun exposure," Oncology Reports; Athens 31, no. 3 (January 2014), http://hdl.handle.net/10342/8210 (accessed December 05, 2020).
Candido, Saverio, Rapisarda, Venerando, Marconi, Andrea, Malaponte, Grazia, Bevelacqua, Valentina, Gangemi, Pietro, Scalisi, Aurora, McCubrey, James A., Maestro, Roberta, Spandidos, Demetrios A., Fenga, Concettina, Libra, Massimo. Analysis of the B-RAFV600E mutation in cutaneous melanoma patients with occupational sun exposure. Oncology Reports; Athens. January 2014; 31(3) 1079-1082. http://hdl.handle.net/10342/8210. Accessed December 05, 2020.