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    Comparison of Whole Blood and Peripheral Blood Mononuclear Cell Gene Expression for Evaluation of the Perioperative Inflammatory Response in Patients with Advanced Heart Failure

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    Bondar, Galyna; Cadeiras, Martin; Wisniewski, Nicholas; Maque, Jetrina; Chittoor, Jay; Chang, Eleanor; Bakir, Maral; Starling, Charlotte; Shahzad, Khurram; Ping, Peipei; Reed, Elaine; Deng, Mario
    Abstract
    Background: Heart failure (HF) prevalence is increasing in the United States. Mechanical Circulatory Support (MCS) therapy is an option for Advanced HF (AdHF) patients. Perioperatively, multiorgan dysfunction (MOD) is linked to the effects of device implantation, augmented by preexisting HF. Early recognition of MOD allows for better diagnosis, treatment, and risk prediction. Gene expression profiling (GEP) was used to evaluate clinical phenotypes of peripheral blood mononuclear cells (PBMC) transcriptomes obtained from patients’ blood samples. Whole blood (WB) samples are clinically more feasible, but their performance in comparison to PBMC samples has not been determined. Methods: We collected blood samples from 31 HF patients (57¡15 years old) undergoing cardiothoracic surgery and 7 healthy age-matched controls, between 2010 and 2011, at a single institution. WB and PBMC samples were collected at a single timepoint postoperatively (median day 8 postoperatively) (25–75% IQR 7–14 days) and subjected to Illumina single color Human BeadChip HT12 v4 whole genome expression array analysis. The Sequential Organ Failure Assessment (SOFA) score was used to characterize the severity of MOD into low (# 4 points), intermediate (5–11), and high ($ 12) risk categories correlating with GEP. Results: Results indicate that the direction of change in GEP of individuals with MOD as compared to controls is similar when determined from PBMC versus WB. The main enriched terms by Gene Ontology (GO) analysis included those involved in the inflammatory response, apoptosis, and other stress response related pathways. The data revealed 35 significant GO categories and 26 pathways overlapping between PBMC and WB. Additionally, class prediction using machine learning tools demonstrated that the subset of significant genes shared by PBMC and WB are sufficient to train as a predictor separating the SOFA groups. Conclusion: GEP analysis of WB has the potential to become a clinical tool for immune-monitoring in patients with MOD
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    http://hdl.handle.net/10342/8214
    Date
    2014-12-17
    Citation:
    APA:
    Bondar, Galyna, & Cadeiras, Martin, & Wisniewski, Nicholas, & Maque, Jetrina, & Chittoor, Jay, & Chang, Eleanor, & Bakir, Maral, & Starling, Charlotte, & Shahzad, Khurram, & Ping, Peipei, & Reed, Elaine, & Deng, Mario. (December 2014). Comparison of Whole Blood and Peripheral Blood Mononuclear Cell Gene Expression for Evaluation of the Perioperative Inflammatory Response in Patients with Advanced Heart Failure. PLOS One, (9:12), p.. Retrieved from http://hdl.handle.net/10342/8214

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    MLA:
    Bondar, Galyna, and Cadeiras, Martin, and Wisniewski, Nicholas, and Maque, Jetrina, and Chittoor, Jay, and Chang, Eleanor, and Bakir, Maral, and Starling, Charlotte, and Shahzad, Khurram, and Ping, Peipei, and Reed, Elaine, and Deng, Mario. "Comparison of Whole Blood and Peripheral Blood Mononuclear Cell Gene Expression for Evaluation of the Perioperative Inflammatory Response in Patients with Advanced Heart Failure". PLOS One. 9:12. (.), December 2014. August 17, 2022. http://hdl.handle.net/10342/8214.
    Chicago:
    Bondar, Galyna and Cadeiras, Martin and Wisniewski, Nicholas and Maque, Jetrina and Chittoor, Jay and Chang, Eleanor and Bakir, Maral and Starling, Charlotte and Shahzad, Khurram and Ping, Peipei and Reed, Elaine and Deng, Mario, "Comparison of Whole Blood and Peripheral Blood Mononuclear Cell Gene Expression for Evaluation of the Perioperative Inflammatory Response in Patients with Advanced Heart Failure," PLOS One 9, no. 12 (December 2014), http://hdl.handle.net/10342/8214 (accessed August 17, 2022).
    AMA:
    Bondar, Galyna, Cadeiras, Martin, Wisniewski, Nicholas, Maque, Jetrina, Chittoor, Jay, Chang, Eleanor, Bakir, Maral, Starling, Charlotte, Shahzad, Khurram, Ping, Peipei, Reed, Elaine, Deng, Mario. Comparison of Whole Blood and Peripheral Blood Mononuclear Cell Gene Expression for Evaluation of the Perioperative Inflammatory Response in Patients with Advanced Heart Failure. PLOS One. December 2014; 9(12) . http://hdl.handle.net/10342/8214. Accessed August 17, 2022.
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