Isolation and molecular identification of endophytic diazotrophs from seeds and stems of three cereal crops
Liu, Huawei; Zhang, Lei; Meng, Aihua; Zhang, Junbiao; Xie, Miaomiao; Qin, Yaohong; Faulk, Dylan Chase; Zhang, Baohong; Yang, Shushen; Qiu, Li
Ten strains of endophytic diazotroph were isolated and identified from the plants collected from three different agricultural crop species, wheat, rice and maize, using the nitrogen-free selective isolation conditions. The nitrogen-fixing ability of endophytic diazotroph was veri- fied by the nifH-PCR assay that showed positive nitrogen fixation ability. These identified strains were classified by 879F-RAPD and 16S rRNA sequence analysis. RAPD analyses revealed that the 10 strains were clustered into seven 879F-RAPD groups, suggesting a clonal origin. 16S rRNA sequencing analyses allowed the assignment of the 10 strains to known groups of nitrogen-fixing bacteria, including organisms from the genera Paenibacil- lus, Enterobacter, Klebsiella and Pantoea. These representative genus are not endophytic diazotrophs in the conventional sense. They may have obtained nitrogen fixation ability through lateral gene transfer, however, the evolutionary forces of lateral gene transfer are not well known. Molecular identification results from 16S rRNA analyses were also con- firmed by morphological and biochemical data. The test strains SH6A and MZB showed positive effect on the growth of plants.
Liu, Huawei, & Zhang, Lei, & Meng, Aihua, & Zhang, Junbiao, & Xie, Miaomiao, & Qin, Yaohong, & Faulk, Dylan Chase, & Zhang, Baohong, & Yang, Shushen, & Qiu, Li. (October 2017). Isolation and molecular identification of endophytic diazotrophs from seeds and stems of three cereal crops. PLOS ONE, (12:10), p.. Retrieved from http://hdl.handle.net/10342/8220
Liu, Huawei, and Zhang, Lei, and Meng, Aihua, and Zhang, Junbiao, and Xie, Miaomiao, and Qin, Yaohong, and Faulk, Dylan Chase, and Zhang, Baohong, and Yang, Shushen, and Qiu, Li. "Isolation and molecular identification of endophytic diazotrophs from seeds and stems of three cereal crops". PLOS ONE. 12:10. (.), October 2017. September 30, 2020. http://hdl.handle.net/10342/8220.
Liu, Huawei and Zhang, Lei and Meng, Aihua and Zhang, Junbiao and Xie, Miaomiao and Qin, Yaohong and Faulk, Dylan Chase and Zhang, Baohong and Yang, Shushen and Qiu, Li, "Isolation and molecular identification of endophytic diazotrophs from seeds and stems of three cereal crops," PLOS ONE 12, no. 10 (October 2017), http://hdl.handle.net/10342/8220 (accessed September 30, 2020).
Liu, Huawei, Zhang, Lei, Meng, Aihua, Zhang, Junbiao, Xie, Miaomiao, Qin, Yaohong, Faulk, Dylan Chase, Zhang, Baohong, Yang, Shushen, Qiu, Li. Isolation and molecular identification of endophytic diazotrophs from seeds and stems of three cereal crops. PLOS ONE. October 2017; 12(10) . http://hdl.handle.net/10342/8220. Accessed September 30, 2020.