• Find People
  • Campus Map
  • PiratePort
  • A-Z
    • About
    • Submit
    • Browse
    • Login
    View Item 
    •   ScholarShip Home
    • Other Campus Research
    • Open Access
    • View Item
    •   ScholarShip Home
    • Other Campus Research
    • Open Access
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of The ScholarShipCommunities & CollectionsDateAuthorsTitlesSubjectsTypeDate SubmittedThis CollectionDateAuthorsTitlesSubjectsTypeDate Submitted

    My Account

    Login

    Statistics

    View Google Analytics Statistics

    Oral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profile

    Thumbnail
    View/ Open
    10.1186-s12866-017-0946-1.pdf (1014.Kb)

    Show full item record
    
    Author
    Silva, Viviam de Oliveira; Pereira, Luciano José; Murata, Ramiro Mendonça
    Abstract
    Background: The aim of this study was to evaluate the effects of β-glucan on the expression of inflammatory mediators and metabolomic profile of oral cells [keratinocytes (OBA-9) and fibroblasts (HGF-1) in a dual-chamber model] infected by Aggregatibacter actinomycetemcomitans. The periodontopathogen was applied and allowed to cross the top layer of cells (OBA-9) to reach the bottom layer of cells (HGF-1) and induce the synthesis of immune factors and cytokines in the host cells. β-glucan (10 μg/mL or 20 μg/mL) were added, and the transcriptional factors and metabolites produced were quantified in the remaining cell layers and supernatant. Results: The relative expression of interleukin (IL)-1-α and IL-18 genes in HGF-1 decreased with 10 μg/mL or 20 μg/mL of β-glucan, where as the expression of PTGS-2 decreased only with 10 μg/mL. The expression of IL-1-α increased with 20 μg/mL and that of IL-18 increased with 10 μg/mL in OBA-9; the expression of BCL 2, EP 300, and PTGS-2 decreased with the higher dose of β-glucan. The production of the metabolite 4-aminobutyric acid presented lower concentrations under 20 μg/mL, whereas the concentrations of 2-deoxytetronic acid NIST and oxalic acid decreased at both concentrations used. Acetophenone, benzoic acid, and pinitol presented reduced concentrations only when treated with 10 μg/mL of β-glucan. Conclusions: Treatment with β-glucans positively modulated the immune response and production of metabolites.
    URI
    http://hdl.handle.net/10342/8242
    Date
    2017-03-07
    Citation:
    APA:
    Silva, Viviam de Oliveira, & Pereira, Luciano José, & Murata, Ramiro Mendonça. (March 2017). Oral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profile. BMC Microbiology, (17:1), p.. Retrieved from http://hdl.handle.net/10342/8242

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Silva, Viviam de Oliveira, and Pereira, Luciano José, and Murata, Ramiro Mendonça. "Oral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profile". BMC Microbiology. 17:1. (.), March 2017. August 15, 2022. http://hdl.handle.net/10342/8242.
    Chicago:
    Silva, Viviam de Oliveira and Pereira, Luciano José and Murata, Ramiro Mendonça, "Oral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profile," BMC Microbiology 17, no. 1 (March 2017), http://hdl.handle.net/10342/8242 (accessed August 15, 2022).
    AMA:
    Silva, Viviam de Oliveira, Pereira, Luciano José, Murata, Ramiro Mendonça. Oral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profile. BMC Microbiology. March 2017; 17(1) . http://hdl.handle.net/10342/8242. Accessed August 15, 2022.
    Collections
    • Open Access

    xmlui.ArtifactBrowser.ItemViewer.elsevier_entitlement

    East Carolina University has created ScholarShip, a digital archive for the scholarly output of the ECU community.

    • About
    • Contact Us
    • Send Feedback