Oral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profile
Author
Silva, Viviam de Oliveira; Pereira, Luciano José; Murata, Ramiro Mendonça
Abstract
Background: The aim of this study was to evaluate the effects of β-glucan on the expression of inflammatory mediators and metabolomic profile of oral cells [keratinocytes (OBA-9) and fibroblasts (HGF-1) in a dual-chamber model] infected by Aggregatibacter actinomycetemcomitans. The periodontopathogen was applied and allowed to cross the top layer of cells (OBA-9) to reach the bottom layer of cells (HGF-1) and induce the synthesis of immune factors and cytokines in the host cells. β-glucan (10 μg/mL or 20 μg/mL) were added, and the transcriptional factors and metabolites produced were quantified in the remaining cell layers and supernatant.
Results: The relative expression of interleukin (IL)-1-α and IL-18 genes in HGF-1 decreased with 10 μg/mL or 20 μg/mL of β-glucan, where as the expression of PTGS-2 decreased only with 10 μg/mL. The expression of IL-1-α increased with 20 μg/mL and that of IL-18 increased with 10 μg/mL in OBA-9; the expression of BCL 2, EP 300, and PTGS-2 decreased with the higher dose of β-glucan. The production of the metabolite 4-aminobutyric acid presented lower concentrations under 20 μg/mL, whereas the concentrations of 2-deoxytetronic acid NIST and oxalic acid decreased at both concentrations used. Acetophenone, benzoic acid, and pinitol presented reduced concentrations only when treated with 10 μg/mL of β-glucan.
Conclusions: Treatment with β-glucans positively modulated the immune response and production of metabolites.
Date
2017-03-07
Citation:
APA:
Silva, Viviam de Oliveira, & Pereira, Luciano José, & Murata, Ramiro Mendonça. (March 2017).
Oral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profile.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/8242
MLA:
Silva, Viviam de Oliveira, and Pereira, Luciano José, and Murata, Ramiro Mendonça.
"Oral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profile". .
. (),
March 2017.
November 30, 2023.
http://hdl.handle.net/10342/8242.
Chicago:
Silva, Viviam de Oliveira and Pereira, Luciano José and Murata, Ramiro Mendonça,
"Oral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profile," , no.
(March 2017),
http://hdl.handle.net/10342/8242 (accessed
November 30, 2023).
AMA:
Silva, Viviam de Oliveira, Pereira, Luciano José, Murata, Ramiro Mendonça.
Oral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profile. .
March 2017;
():
.
http://hdl.handle.net/10342/8242. Accessed
November 30, 2023.
Collections