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    Quantitative analysis and comparison of 3D morphology between viable and apoptotic MCF-7 breast cancer cells and characterization of nuclear fragmentation

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    Author
    Wen, Yuhua; Chen, Zhan; Lu, Jianfen; Ables, Elizabeth; Scemama, Jean-Luc; Yang, Li V.; Lu, Jun Q.; Hu, Xin-Hua
    Abstract
    Morphological changes in apoptotic cells provide essential markers for defining and detec- tion of apoptosis as a fundamental mechanism of cell death. Among these changes, the nuclear fragmentation and condensation have been regarded as the important markers but quantitative characterization of these changes is yet to be achieved. We have acquired con- focal image stacks of 206 viable and apoptotic MCF-7 cells stained by three fluorescent dyes. Three-dimensional (3D) parameters were extracted to quantify and compare their dif- ferences in morphology. To analyze nuclear fragmentation, a new method has been devel- oped to determine clustering of nuclear voxels in the reconstructed cells due to fluorescence intensity changes in nuclei of apoptotic cells. The results of these studies reveal that the 3D morphological changes in cytoplasm and nuclear membranes in apoptotic cells provide sen- sitive targets for label-free detection and staging of apoptosis. Furthermore, the clustering analysis and morphological data on nuclear fragmentation are highly useful for derivation of optical cell models and simulation of diffraction images to investigate light scattering by early apoptotic cells, which can lead to future development of label-free and rapid methods of apoptosis assay based on cell morphology.
    URI
    http://hdl.handle.net/10342/8246
    Date
    2017-09-08
    Citation:
    APA:
    Wen, Yuhua, & Chen, Zhan, & Lu, Jianfen, & Ables, Elizabeth, & Scemama, Jean-Luc, & Yang, Li V., & Lu, Jun Q., & Hu, Xin-Hua. (September 2017). Quantitative analysis and comparison of 3D morphology between viable and apoptotic MCF-7 breast cancer cells and characterization of nuclear fragmentation. PLoS One, (12:9), p.. Retrieved from http://hdl.handle.net/10342/8246

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    MLA:
    Wen, Yuhua, and Chen, Zhan, and Lu, Jianfen, and Ables, Elizabeth, and Scemama, Jean-Luc, and Yang, Li V., and Lu, Jun Q., and Hu, Xin-Hua. "Quantitative analysis and comparison of 3D morphology between viable and apoptotic MCF-7 breast cancer cells and characterization of nuclear fragmentation". PLoS One. 12:9. (.), September 2017. March 06, 2021. http://hdl.handle.net/10342/8246.
    Chicago:
    Wen, Yuhua and Chen, Zhan and Lu, Jianfen and Ables, Elizabeth and Scemama, Jean-Luc and Yang, Li V. and Lu, Jun Q. and Hu, Xin-Hua, "Quantitative analysis and comparison of 3D morphology between viable and apoptotic MCF-7 breast cancer cells and characterization of nuclear fragmentation," PLoS One 12, no. 9 (September 2017), http://hdl.handle.net/10342/8246 (accessed March 06, 2021).
    AMA:
    Wen, Yuhua, Chen, Zhan, Lu, Jianfen, Ables, Elizabeth, Scemama, Jean-Luc, Yang, Li V., Lu, Jun Q., Hu, Xin-Hua. Quantitative analysis and comparison of 3D morphology between viable and apoptotic MCF-7 breast cancer cells and characterization of nuclear fragmentation. PLoS One. September 2017; 12(9) . http://hdl.handle.net/10342/8246. Accessed March 06, 2021.
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