Dose-Dependent Effects of GLD-2 and GLD-1 on Germline Differentiation and Dedifferentiation in the Absence of PUF-8
Author
Park, Youngyong; O’Rourke, Samuel; Taki, Faten A.; Alfhili, Mohammad A.; Hee Lee, Myon
Abstract
PUMILIO/FBF (PUF) proteins have a conserved function in stem cell regulation.
Caenorhabditis elegans PUF-8 protein inhibits the translation of target mRNAs by
interacting with PUF binding element (PBE) in the 30 untranslated region (30 UTR). In
this work, an in silico analysis has identified gld-2 [a poly(A) polymerase] as a putative
PUF-8 target. Biochemical and reporter analyses showed that PUF-8 specifically binds
to a PBE in gld-2 3
0 UTR and represses a GFP reporter gene carrying gld-2 3
0 UTR
in the C. elegans mitotic germ cells. GLD-2 enhances meiotic entry at least in part by
activating GLD-1 (a KH motif-containing RNA-binding protein). Our genetic analyses
also demonstrated that heterozygous gld-2(+/−) gld-1(+/−) genes in the absence
of PUF-8 are competent for meiotic entry (early differentiation), but haplo-insufficient
for the meiotic division (terminal differentiation) of spermatocytes. Indeed, the arrested
spermatocytes return to mitotic cells via dedifferentiation, which results in germline
tumors. Since these regulators are broadly conserved, we thus suggest that similar
molecular mechanisms may control differentiation, dedifferentiation, and tumorigenesis
in other organisms, including humans.
Date
2020-01-24
Citation:
APA:
Park, Youngyong, & O’Rourke, Samuel, & Taki, Faten A., & Alfhili, Mohammad A., & Hee Lee, Myon. (January 2020).
Dose-Dependent Effects of GLD-2 and GLD-1 on Germline Differentiation and Dedifferentiation in the Absence of PUF-8.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/8310
MLA:
Park, Youngyong, and O’Rourke, Samuel, and Taki, Faten A., and Alfhili, Mohammad A., and Hee Lee, Myon.
"Dose-Dependent Effects of GLD-2 and GLD-1 on Germline Differentiation and Dedifferentiation in the Absence of PUF-8". .
. (),
January 2020.
December 04, 2023.
http://hdl.handle.net/10342/8310.
Chicago:
Park, Youngyong and O’Rourke, Samuel and Taki, Faten A. and Alfhili, Mohammad A. and Hee Lee, Myon,
"Dose-Dependent Effects of GLD-2 and GLD-1 on Germline Differentiation and Dedifferentiation in the Absence of PUF-8," , no.
(January 2020),
http://hdl.handle.net/10342/8310 (accessed
December 04, 2023).
AMA:
Park, Youngyong, O’Rourke, Samuel, Taki, Faten A., Alfhili, Mohammad A., Hee Lee, Myon.
Dose-Dependent Effects of GLD-2 and GLD-1 on Germline Differentiation and Dedifferentiation in the Absence of PUF-8. .
January 2020;
():
.
http://hdl.handle.net/10342/8310. Accessed
December 04, 2023.
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