Small RNA Sequencing Reveals Regulatory Roles of MicroRNAs in the Development of Meloidogyne incognita
Author
Liu, Huawei; Nichols, Robert L.; Qiu, Li; Sun, Runrun; Zhang, Baohong; Pan, Xiaoping
Abstract
MicroRNAs (miRNAs) are an extensive class of small regulatory RNAs. Knowing the
specific expression and functions of miRNAs during root-knot nematode (RKN) (Meloidogyne incognita)
development could provide fundamental information about RKN development as well as a means to
design new strategies to control RKN infection, a major problem of many important crops. Employing
high throughput deep sequencing, we identified a total of 45 conserved and novel miRNAs from
two developmental stages of RKN, eggs and J2 juveniles, during their infection of cotton (Gossypium
hirsutum L.). Twenty-one of the miRNAs were differentially expressed between the two stages.
Compared with their expression in eggs, two miRNAs were upregulated (miR252 and miRN19),
whereas 19 miRNAs were downregulated in J2 juveniles. Nine miRNAs were expressed at high
levels, with >1000 reads per mapped million (RPM) sequenced reads in both eggs and J2 juveniles
(miR1, miR124, miR2-3p, miR252, miR279, miR57-5p, miR7904, miR87, and miR92). Three miRNAs
were only expressed in eggs (miR4738, miRN3, and miRN5). These differentially expressed miRNAs
may control RKN development by regulating specific protein-coding genes in pathways associated
with RKN growth and development.
Date
2019-11-02
Citation:
APA:
Liu, Huawei, & Nichols, Robert L., & Qiu, Li, & Sun, Runrun, & Zhang, Baohong, & Pan, Xiaoping. (November 2019).
Small RNA Sequencing Reveals Regulatory Roles of MicroRNAs in the Development of Meloidogyne incognita.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/8323
MLA:
Liu, Huawei, and Nichols, Robert L., and Qiu, Li, and Sun, Runrun, and Zhang, Baohong, and Pan, Xiaoping.
"Small RNA Sequencing Reveals Regulatory Roles of MicroRNAs in the Development of Meloidogyne incognita". .
. (),
November 2019.
September 30, 2023.
http://hdl.handle.net/10342/8323.
Chicago:
Liu, Huawei and Nichols, Robert L. and Qiu, Li and Sun, Runrun and Zhang, Baohong and Pan, Xiaoping,
"Small RNA Sequencing Reveals Regulatory Roles of MicroRNAs in the Development of Meloidogyne incognita," , no.
(November 2019),
http://hdl.handle.net/10342/8323 (accessed
September 30, 2023).
AMA:
Liu, Huawei, Nichols, Robert L., Qiu, Li, Sun, Runrun, Zhang, Baohong, Pan, Xiaoping.
Small RNA Sequencing Reveals Regulatory Roles of MicroRNAs in the Development of Meloidogyne incognita. .
November 2019;
():
.
http://hdl.handle.net/10342/8323. Accessed
September 30, 2023.
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