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    Small RNA Sequencing Reveals Regulatory Roles of MicroRNAs in the Development of Meloidogyne incognita

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    Author
    Liu, Huawei; Nichols, Robert L.; Qiu, Li; Sun, Runrun; Zhang, Baohong; Pan, Xiaoping
    Abstract
    MicroRNAs (miRNAs) are an extensive class of small regulatory RNAs. Knowing the specific expression and functions of miRNAs during root-knot nematode (RKN) (Meloidogyne incognita) development could provide fundamental information about RKN development as well as a means to design new strategies to control RKN infection, a major problem of many important crops. Employing high throughput deep sequencing, we identified a total of 45 conserved and novel miRNAs from two developmental stages of RKN, eggs and J2 juveniles, during their infection of cotton (Gossypium hirsutum L.). Twenty-one of the miRNAs were differentially expressed between the two stages. Compared with their expression in eggs, two miRNAs were upregulated (miR252 and miRN19), whereas 19 miRNAs were downregulated in J2 juveniles. Nine miRNAs were expressed at high levels, with >1000 reads per mapped million (RPM) sequenced reads in both eggs and J2 juveniles (miR1, miR124, miR2-3p, miR252, miR279, miR57-5p, miR7904, miR87, and miR92). Three miRNAs were only expressed in eggs (miR4738, miRN3, and miRN5). These differentially expressed miRNAs may control RKN development by regulating specific protein-coding genes in pathways associated with RKN growth and development.
    URI
    http://hdl.handle.net/10342/8323
    Subject
    root-knot nematode; cotton infection; small RNA; gene regulation; deep sequencing
    Date
    2019-11-02
    Citation:
    APA:
    Liu, Huawei, & Nichols, Robert L., & Qiu, Li, & Sun, Runrun, & Zhang, Baohong, & Pan, Xiaoping. (November 2019). Small RNA Sequencing Reveals Regulatory Roles of MicroRNAs in the Development of Meloidogyne incognita. International Journal of Molecular Sciences, (20:21), p.5466. Retrieved from http://hdl.handle.net/10342/8323

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Liu, Huawei, and Nichols, Robert L., and Qiu, Li, and Sun, Runrun, and Zhang, Baohong, and Pan, Xiaoping. "Small RNA Sequencing Reveals Regulatory Roles of MicroRNAs in the Development of Meloidogyne incognita". International Journal of Molecular Sciences. 20:21. (5466.), November 2019. March 03, 2021. http://hdl.handle.net/10342/8323.
    Chicago:
    Liu, Huawei and Nichols, Robert L. and Qiu, Li and Sun, Runrun and Zhang, Baohong and Pan, Xiaoping, "Small RNA Sequencing Reveals Regulatory Roles of MicroRNAs in the Development of Meloidogyne incognita," International Journal of Molecular Sciences 20, no. 21 (November 2019), http://hdl.handle.net/10342/8323 (accessed March 03, 2021).
    AMA:
    Liu, Huawei, Nichols, Robert L., Qiu, Li, Sun, Runrun, Zhang, Baohong, Pan, Xiaoping. Small RNA Sequencing Reveals Regulatory Roles of MicroRNAs in the Development of Meloidogyne incognita. International Journal of Molecular Sciences. November 2019; 20(21) 5466. http://hdl.handle.net/10342/8323. Accessed March 03, 2021.
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