• Find People
  • Campus Map
  • PiratePort
  • A-Z
    • About
    • Submit
    • Browse
    • Login
    View Item 
    •   ScholarShip Home
    • Other Campus Research
    • Open Access
    • View Item
    •   ScholarShip Home
    • Other Campus Research
    • Open Access
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of The ScholarShipCommunities & CollectionsDateAuthorsTitlesSubjectsTypeDate SubmittedThis CollectionDateAuthorsTitlesSubjectsTypeDate Submitted

    My Account

    Login

    Statistics

    View Google Analytics Statistics

    Species-Specific Conservation of Linear Antigenic Sites on Vaccinia Virus A27 Protein Homologs of Orthopoxviruses

    Thumbnail
    View/ Open
    viruses-11-00493.pdf (2.153Mb)

    Show full item record
    Author
    Ahsendorf, Henrike P.; Gan, Li L.; Eltom, Kamal H.; Wahed, Ahmed Abd El; Hotop, Sven-Kevin; Roper, Rachel L.; Beutling, Ulrike; Broenstrup, Mark; Stahl-Hennig, Christiane; Hoelzle, Ludwig E.; Czerny, Claus-Peter
    Abstract
    The vaccinia virus (VACV) A27 protein and its homologs, which are found in a large number of members of the genus Orthopoxvirus (OPXV), are targets of viral neutralization by host antibodies. We have mapped six binding sites (epitopes #1A: aa 32–39, #1B: aa 28–33, #1C: aa 26–31, #1D: 28–34, #4: aa 9–14, and #5: aa 68–71) of A27 specific monoclonal antibodies (mAbs) using peptide arrays. MAbs recognizing epitopes #1A–D and #4 neutralized VACV Elstree in a complement dependent way (50% plaque-reduction: 12.5–200 µg/mL). Fusion of VACV at low pH was blocked through inhibition of epitope #1A. To determine the sequence variability of the six antigenic sites, 391 sequences of A27 protein homologs available were compared. Epitopes #4 and #5 were conserved among most of the OPXVs, while the sequential epitope complex #1A–D was more variable and, therefore, responsible for species-specific epitope characteristics. The accurate and reliable mapping of defined epitopes on immuno-protective proteins such as the A27 of VACV enables phylogenetic studies and insights into OPXV evolution as well as to pave the way to the development of safer vaccines and chemical or biological antivirals.
    URI
    http://hdl.handle.net/10342/8331
    Subject
    Vaccinia virus A27 protein homologs; epitope mapping; phylogenetic epitope variation; neutralizing antibodies
    Date
    2019-05-29
    Citation:
    APA:
    Ahsendorf, Henrike P., & Gan, Li L., & Eltom, Kamal H., & Wahed, Ahmed Abd El, & Hotop, Sven-Kevin, & Roper, Rachel L., & Beutling, Ulrike, & Broenstrup, Mark, & Stahl-Hennig, Christiane, & Hoelzle, Ludwig E., & Czerny, Claus-Peter. (May 2019). Species-Specific Conservation of Linear Antigenic Sites on Vaccinia Virus A27 Protein Homologs of Orthopoxviruses. , (), - . Retrieved from http://hdl.handle.net/10342/8331

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Ahsendorf, Henrike P., and Gan, Li L., and Eltom, Kamal H., and Wahed, Ahmed Abd El, and Hotop, Sven-Kevin, and Roper, Rachel L., and Beutling, Ulrike, and Broenstrup, Mark, and Stahl-Hennig, Christiane, and Hoelzle, Ludwig E., and Czerny, Claus-Peter. "Species-Specific Conservation of Linear Antigenic Sites on Vaccinia Virus A27 Protein Homologs of Orthopoxviruses". . . (), May 2019. September 26, 2023. http://hdl.handle.net/10342/8331.
    Chicago:
    Ahsendorf, Henrike P. and Gan, Li L. and Eltom, Kamal H. and Wahed, Ahmed Abd El and Hotop, Sven-Kevin and Roper, Rachel L. and Beutling, Ulrike and Broenstrup, Mark and Stahl-Hennig, Christiane and Hoelzle, Ludwig E. and Czerny, Claus-Peter, "Species-Specific Conservation of Linear Antigenic Sites on Vaccinia Virus A27 Protein Homologs of Orthopoxviruses," , no. (May 2019), http://hdl.handle.net/10342/8331 (accessed September 26, 2023).
    AMA:
    Ahsendorf, Henrike P., Gan, Li L., Eltom, Kamal H., Wahed, Ahmed Abd El, Hotop, Sven-Kevin, Roper, Rachel L., Beutling, Ulrike, Broenstrup, Mark, Stahl-Hennig, Christiane, Hoelzle, Ludwig E., Czerny, Claus-Peter. Species-Specific Conservation of Linear Antigenic Sites on Vaccinia Virus A27 Protein Homologs of Orthopoxviruses. . May 2019; (): . http://hdl.handle.net/10342/8331. Accessed September 26, 2023.
    Collections
    • Open Access

    xmlui.ArtifactBrowser.ItemViewer.elsevier_entitlement

    East Carolina University has created ScholarShip, a digital archive for the scholarly output of the ECU community.

    • About
    • Contact Us
    • Send Feedback