Species-Specific Conservation of Linear Antigenic Sites on Vaccinia Virus A27 Protein Homologs of Orthopoxviruses
Author
Ahsendorf, Henrike P.; Gan, Li L.; Eltom, Kamal H.; Wahed, Ahmed Abd El; Hotop, Sven-Kevin; Roper, Rachel L.; Beutling, Ulrike; Broenstrup, Mark; Stahl-Hennig, Christiane; Hoelzle, Ludwig E.; Czerny, Claus-Peter
Abstract
The vaccinia virus (VACV) A27 protein and its homologs, which are found in a large number of members of the genus Orthopoxvirus (OPXV), are targets of viral neutralization by host antibodies. We have mapped six binding sites (epitopes #1A: aa 32–39, #1B: aa 28–33, #1C: aa 26–31, #1D: 28–34, #4: aa 9–14, and #5: aa 68–71) of A27 specific monoclonal antibodies (mAbs) using peptide arrays. MAbs recognizing epitopes #1A–D and #4 neutralized VACV Elstree in a complement dependent way (50% plaque-reduction: 12.5–200 µg/mL). Fusion of VACV at low pH was blocked through inhibition of epitope #1A. To determine the sequence variability of the six antigenic sites, 391 sequences of A27 protein homologs available were compared. Epitopes #4 and #5 were conserved among most of the OPXVs, while the sequential epitope complex #1A–D was more variable and, therefore, responsible for species-specific epitope characteristics. The accurate and reliable mapping of defined epitopes on immuno-protective proteins such as the A27 of VACV enables phylogenetic studies and insights into OPXV evolution as well as to pave the way to the development of safer vaccines and chemical or biological antivirals.
Date
2019-05-29
Citation:
APA:
Ahsendorf, Henrike P., & Gan, Li L., & Eltom, Kamal H., & Wahed, Ahmed Abd El, & Hotop, Sven-Kevin, & Roper, Rachel L., & Beutling, Ulrike, & Broenstrup, Mark, & Stahl-Hennig, Christiane, & Hoelzle, Ludwig E., & Czerny, Claus-Peter. (May 2019).
Species-Specific Conservation of Linear Antigenic Sites on Vaccinia Virus A27 Protein Homologs of Orthopoxviruses.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/8331
MLA:
Ahsendorf, Henrike P., and Gan, Li L., and Eltom, Kamal H., and Wahed, Ahmed Abd El, and Hotop, Sven-Kevin, and Roper, Rachel L., and Beutling, Ulrike, and Broenstrup, Mark, and Stahl-Hennig, Christiane, and Hoelzle, Ludwig E., and Czerny, Claus-Peter.
"Species-Specific Conservation of Linear Antigenic Sites on Vaccinia Virus A27 Protein Homologs of Orthopoxviruses". .
. (),
May 2019.
September 26, 2023.
http://hdl.handle.net/10342/8331.
Chicago:
Ahsendorf, Henrike P. and Gan, Li L. and Eltom, Kamal H. and Wahed, Ahmed Abd El and Hotop, Sven-Kevin and Roper, Rachel L. and Beutling, Ulrike and Broenstrup, Mark and Stahl-Hennig, Christiane and Hoelzle, Ludwig E. and Czerny, Claus-Peter,
"Species-Specific Conservation of Linear Antigenic Sites on Vaccinia Virus A27 Protein Homologs of Orthopoxviruses," , no.
(May 2019),
http://hdl.handle.net/10342/8331 (accessed
September 26, 2023).
AMA:
Ahsendorf, Henrike P., Gan, Li L., Eltom, Kamal H., Wahed, Ahmed Abd El, Hotop, Sven-Kevin, Roper, Rachel L., Beutling, Ulrike, Broenstrup, Mark, Stahl-Hennig, Christiane, Hoelzle, Ludwig E., Czerny, Claus-Peter.
Species-Specific Conservation of Linear Antigenic Sites on Vaccinia Virus A27 Protein Homologs of Orthopoxviruses. .
May 2019;
():
.
http://hdl.handle.net/10342/8331. Accessed
September 26, 2023.
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