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    The proteome of extracellular vesicles released by clastic cells differs based on their substrate

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    Author
    Rody, Wellington J. Jr.; Chamberlain, Casey A.; Emory-Carter, Alyssa K.; McHugh, Kevin P.; Wallet, Shannon M.; Spicer, Victor; Krokhin, Oleg; Holliday, L. Shannon
    Abstract
    Extracellular vesicles (EVs) from osteoclasts are important regulators in intercellular communication. Here, we investigated the proteome of EVs from clastic cells plated on plastic (clasts), bone (osteoclasts) and dentin (odontoclasts) by two-dimensional high performance liquid chromatography mass spectrometry seeking differences attributable to distinct mineralized matrices. A total of 1,952 proteins were identified. Of the 500 most abundant proteins in EVs, osteoclast and odontoclast EVs were 83.3% identical, while clasts shared 70.7% of the proteins with osteoclasts and 74.2% of proteins with odontoclasts. For each protein, the differences between the total ion count values were mapped to an expression ratio histogram (Z-score) in order to detect proteins differentially expressed. Stabilin-1 and macrophage mannose receptor-1 were significantly-enriched in EVs from odontoclasts compared with osteoclasts (Z = 2.45, Z = 3.34) and clasts (Z = 13.86, Z = 1.81) and were abundant in odontoclast EVs. Numerous less abundant proteins were differentially-enriched. Subunits of known protein complexes were abundant in clastic EVs, and were present at levels consistent with them being in assembled protein complexes. These included the proteasome, COP1, COP9, the T complex and a novel sub-complex of vacuolar H+ -ATPase (V-ATPase), which included the (pro) renin receptor. The (pro) renin receptor was immunoprecipitated using an anti-E-subunit antibody from detergent-solubilized EVs, supporting the idea that the V-ATPase subunits present were in the same protein complex. We conclude that the protein composition of EVs released by clastic cells changes based on the substrate. Clastic EVs are enriched in various protein complexes including a previously undescribed VATPase sub-complex.
    URI
    http://hdl.handle.net/10342/8349
    Date
    2019-07-10
    Citation:
    APA:
    Rody, Wellington J. Jr., & Chamberlain, Casey A., & Emory-Carter, Alyssa K., & McHugh, Kevin P., & Wallet, Shannon M., & Spicer, Victor, & Krokhin, Oleg, & Holliday, L. Shannon. (July 2019). The proteome of extracellular vesicles released by clastic cells differs based on their substrate. PLOS ONE, (14:7), p.e0219602. Retrieved from http://hdl.handle.net/10342/8349

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    MLA:
    Rody, Wellington J. Jr., and Chamberlain, Casey A., and Emory-Carter, Alyssa K., and McHugh, Kevin P., and Wallet, Shannon M., and Spicer, Victor, and Krokhin, Oleg, and Holliday, L. Shannon. "The proteome of extracellular vesicles released by clastic cells differs based on their substrate". PLOS ONE. 14:7. (e0219602.), July 2019. August 11, 2022. http://hdl.handle.net/10342/8349.
    Chicago:
    Rody, Wellington J. Jr. and Chamberlain, Casey A. and Emory-Carter, Alyssa K. and McHugh, Kevin P. and Wallet, Shannon M. and Spicer, Victor and Krokhin, Oleg and Holliday, L. Shannon, "The proteome of extracellular vesicles released by clastic cells differs based on their substrate," PLOS ONE 14, no. 7 (July 2019), http://hdl.handle.net/10342/8349 (accessed August 11, 2022).
    AMA:
    Rody, Wellington J. Jr., Chamberlain, Casey A., Emory-Carter, Alyssa K., McHugh, Kevin P., Wallet, Shannon M., Spicer, Victor, Krokhin, Oleg, Holliday, L. Shannon. The proteome of extracellular vesicles released by clastic cells differs based on their substrate. PLOS ONE. July 2019; 14(7) e0219602. http://hdl.handle.net/10342/8349. Accessed August 11, 2022.
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