RhoGTPase Regulators Orchestrate Distinct Stages of Synaptic Development
Martin-Vilchez, Samuel; Whitmore, Leanna; Asmussen, Hannelore; Zareno, Jessica; Horwitz, Rick; Newell-Litwa, Karen
Small RhoGTPases regulate changes in post-synaptic spine morphology and density that support learning and memory. They are also major targets of synaptic disorders, including Autism. Here we sought to determine whether upstream RhoGTPase regulators, including GEFs, GAPs, and GDIs, sculpt specific stages of synaptic development. The majority of examined molecules uniquely regulate either early spine precursor formation or later matura- tion. Specifically, an activator of actin polymerization, the Rac1 GEF β-PIX, drives spine pre- cursor formation, whereas both FRABIN, a Cdc42 GEF, and OLIGOPHRENIN-1, a RhoA GAP, regulate spine precursor elongation. However, in later development, a novel Rac1 GAP, ARHGAP23, and RhoGDIs inactivate actomyosin dynamics to stabilize mature synap- ses. Our observations demonstrate that specific combinations of RhoGTPase regulatory pro- teins temporally balance RhoGTPase activity during post-synaptic spine development.
Martin-Vilchez, Samuel, & Whitmore, Leanna, & Asmussen, Hannelore, & Zareno, Jessica, & Horwitz, Rick, & Newell-Litwa, Karen. (January 2017). RhoGTPase Regulators Orchestrate Distinct Stages of Synaptic Development. PLoS One, (12:1), p.. Retrieved from http://hdl.handle.net/10342/8354
Martin-Vilchez, Samuel, and Whitmore, Leanna, and Asmussen, Hannelore, and Zareno, Jessica, and Horwitz, Rick, and Newell-Litwa, Karen. "RhoGTPase Regulators Orchestrate Distinct Stages of Synaptic Development". PLoS One. 12:1. (.), January 2017. September 20, 2020. http://hdl.handle.net/10342/8354.
Martin-Vilchez, Samuel and Whitmore, Leanna and Asmussen, Hannelore and Zareno, Jessica and Horwitz, Rick and Newell-Litwa, Karen, "RhoGTPase Regulators Orchestrate Distinct Stages of Synaptic Development," PLoS One 12, no. 1 (January 2017), http://hdl.handle.net/10342/8354 (accessed September 20, 2020).
Martin-Vilchez, Samuel, Whitmore, Leanna, Asmussen, Hannelore, Zareno, Jessica, Horwitz, Rick, Newell-Litwa, Karen. RhoGTPase Regulators Orchestrate Distinct Stages of Synaptic Development. PLoS One. January 2017; 12(1) . http://hdl.handle.net/10342/8354. Accessed September 20, 2020.