• Find People
  • Campus Map
  • PiratePort
  • A-Z
    • About
    • Submit
    • Browse
    • Login
    View Item 
    •   ScholarShip Home
    • Other Campus Research
    • Open Access
    • View Item
    •   ScholarShip Home
    • Other Campus Research
    • Open Access
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of The ScholarShipCommunities & CollectionsDateAuthorsTitlesSubjectsTypeDate SubmittedThis CollectionDateAuthorsTitlesSubjectsTypeDate Submitted

    My Account

    Login

    Statistics

    View Google Analytics Statistics

    Advanced immunostaining approaches to study early male germ cell development

    Thumbnail
    View/ Open
    1-s2.0-S1873506118300370-main.pdf (3.356Mb)

    Show full item record
    Author
    Niedenberger, Bryan A.; Geyer, Christopher B.
    Abstract
    Mammalian male germ cell development takes place in the testis under the influence of a variety of somatic cells and an incompletely defined paracrine and endocrine influences. Since it is not recapitulated well in vitro, researchers studying spermatogenesis often manipulate the germline by creating transgenic or knockout mice or by administering pharmaceutical agonists/antagonists or inhibitors. The effects of these types of manipulations on germline development can often be determined following microscopic imaging, both of stained and immunostained testis sections. Here, we describe approaches for microscopic analysis of the developing male germline, provide detailed protocols for a variety of immunostaining approaches, and discuss transgenic fluorescent reporter lines for studying the early stages of spermatogenesis.
    URI
    http://hdl.handle.net/10342/8356
    Subject
    Prospermatogonia; Spermatogonia; Testis; Immunofluorescence; Immunohistochemistry
    Date
    2018-02-09
    Citation:
    APA:
    Niedenberger, Bryan A., & Geyer, Christopher B.. (February 2018). Advanced immunostaining approaches to study early male germ cell development. , (), - . Retrieved from http://hdl.handle.net/10342/8356

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Niedenberger, Bryan A., and Geyer, Christopher B.. "Advanced immunostaining approaches to study early male germ cell development". . . (), February 2018. December 04, 2023. http://hdl.handle.net/10342/8356.
    Chicago:
    Niedenberger, Bryan A. and Geyer, Christopher B., "Advanced immunostaining approaches to study early male germ cell development," , no. (February 2018), http://hdl.handle.net/10342/8356 (accessed December 04, 2023).
    AMA:
    Niedenberger, Bryan A., Geyer, Christopher B.. Advanced immunostaining approaches to study early male germ cell development. . February 2018; (): . http://hdl.handle.net/10342/8356. Accessed December 04, 2023.
    Collections
    • Open Access

    xmlui.ArtifactBrowser.ItemViewer.elsevier_entitlement

    East Carolina University has created ScholarShip, a digital archive for the scholarly output of the ECU community.

    • About
    • Contact Us
    • Send Feedback