Sorbus alnifolia protects dopaminergic neurodegeneration in Caenorhabditis elegans
Cheon, Se-Myeong; Jang, Insoo; Lee, Myon-Hee; Kim, Dae Keun; Jeon, Hoon; Cha, Dong Seok
Context: The twigs of Sorbus alnifolia (Sieb. et Zucc.) K. Koch (Rosaceae) have been used to treat neuro- logical disorders as a traditional medicine in Korea. However, there are limited data describing the efficacy of S. alnifolia in Parkinson’s disease (PD). Objective: This study was conducted to identify the protective effects of the methanol extracts of S. alnifolia (MESA) on the dopaminergic (DA) neurodegeneration in Caenorhabditis elegans. Materials and methods: To test the neuroprotective action of MESA, viability assay was performed after 48 h exposure to 1-methyl-4-phenylpyridine (MMPþ) in PC12 cells and C. elegans (400 lM and 2 mM of MMPþ, respectively). Fluorescence intensity was quantified using transgenic mutants such as BZ555 (Pdat-1::GFP) and and UA57 (Pdat-1::GFP and Pdat-1::CAT-2) to determine MESA’s effects on DA neurode- generation in C. elegans. Aggregation of a-synuclein was observed using NL5901 strain (unc-54p::a- synuclein::YFP). MESA’s protective effects on the DA neuronal functions were examined by food-sensing assay. Lifespan assay was conducted to test the effects of MESA on the longevity. Results: MESA restored MPPþ-induced loss of viability in both PC12 cells and C. elegans (85.8% and 54.9%, respectively). In C. elegans, MESA provided protection against chemically and genetically-induced DA neurodegeneration, respectively. Moreover, food-sensing functions were increased 58.4% by MESA in the DA neuron degraded worms. MESA also prolonged the average lifespan by 25.6%. However, MESA failed to alter a-synuclein aggregation. Discussion and conclusions: These results revealed that MESA protects DA neurodegeneration and recov- ers diminished DA neuronal functions, thereby can be a valuable candidate for the treatment of PD.
Cheon, Se-Myeong, & Jang, Insoo, & Lee, Myon-Hee, & Kim, Dae Keun, & Jeon, Hoon, & Cha, Dong Seok. (January 2017). Sorbus alnifolia protects dopaminergic neurodegeneration in Caenorhabditis elegans. Pharmaceutical Biology, (55:1), p.481–486. Retrieved from http://hdl.handle.net/10342/8359
Cheon, Se-Myeong, and Jang, Insoo, and Lee, Myon-Hee, and Kim, Dae Keun, and Jeon, Hoon, and Cha, Dong Seok. "Sorbus alnifolia protects dopaminergic neurodegeneration in Caenorhabditis elegans". Pharmaceutical Biology. 55:1. (481–486.), January 2017. September 23, 2020. http://hdl.handle.net/10342/8359.
Cheon, Se-Myeong and Jang, Insoo and Lee, Myon-Hee and Kim, Dae Keun and Jeon, Hoon and Cha, Dong Seok, "Sorbus alnifolia protects dopaminergic neurodegeneration in Caenorhabditis elegans," Pharmaceutical Biology 55, no. 1 (January 2017), http://hdl.handle.net/10342/8359 (accessed September 23, 2020).
Cheon, Se-Myeong, Jang, Insoo, Lee, Myon-Hee, Kim, Dae Keun, Jeon, Hoon, Cha, Dong Seok. Sorbus alnifolia protects dopaminergic neurodegeneration in Caenorhabditis elegans. Pharmaceutical Biology. January 2017; 55(1) 481–486. http://hdl.handle.net/10342/8359. Accessed September 23, 2020.