Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum
Author
Liu, Yuling; Zhang, Baohong; Wen, Xinpeng; Zhang, Shulin; Wei, Yangyang; Lu, Quanwei; Liu, Zhen; Wang, Kunbo; Liu, Fang; Peng, Renhai
Abstract
A bacterial artificial chromosome (BAC) library for G. mustelinum Miers ex G. Watt (AD4)
was constructed. Intact nuclei from G. mustelinum (AD4) were used to isolate high molecular
weight DNA, which was partially cleaved with Hind III and cloned into pSMART BAC (Hind
III) vectors. The BAC library consisted of 208,182 clones arrayed in 542 384-microtiter
plates, with an average insert size of 121.72 kb ranging from 100 to 150 kb. About 2% of the
clones did not contain inserts. Based on an estimated genome size of 2372 Mb for G. mustelinum, the BAC library was estimated to have a total coverage of 10.50 × genome equivalents. The high capacity library of G. mustelinum will serve as a giant gene resource for
map-based cloning of quantitative trait loci or genes associated with important agronomic
traits or resistance to Verticillium wilt, physical mapping and comparative genome analysis.
Date
2018-05-17
Citation:
APA:
Liu, Yuling, & Zhang, Baohong, & Wen, Xinpeng, & Zhang, Shulin, & Wei, Yangyang, & Lu, Quanwei, & Liu, Zhen, & Wang, Kunbo, & Liu, Fang, & Peng, Renhai. (May 2018).
Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/8417
MLA:
Liu, Yuling, and Zhang, Baohong, and Wen, Xinpeng, and Zhang, Shulin, and Wei, Yangyang, and Lu, Quanwei, and Liu, Zhen, and Wang, Kunbo, and Liu, Fang, and Peng, Renhai.
"Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum". .
. (),
May 2018.
March 19, 2024.
http://hdl.handle.net/10342/8417.
Chicago:
Liu, Yuling and Zhang, Baohong and Wen, Xinpeng and Zhang, Shulin and Wei, Yangyang and Lu, Quanwei and Liu, Zhen and Wang, Kunbo and Liu, Fang and Peng, Renhai,
"Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum," , no.
(May 2018),
http://hdl.handle.net/10342/8417 (accessed
March 19, 2024).
AMA:
Liu, Yuling, Zhang, Baohong, Wen, Xinpeng, Zhang, Shulin, Wei, Yangyang, Lu, Quanwei, Liu, Zhen, Wang, Kunbo, Liu, Fang, Peng, Renhai.
Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum. .
May 2018;
():
.
http://hdl.handle.net/10342/8417. Accessed
March 19, 2024.
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