Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum

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Author
Liu, Yuling; Zhang, Baohong; Wen, Xinpeng; Zhang, Shulin; Wei, Yangyang; Lu, Quanwei; Liu, Zhen; Wang, Kunbo; Liu, Fang; Peng, Renhai
Abstract
A bacterial artificial chromosome (BAC) library for G. mustelinum Miers ex G. Watt (AD4) was constructed. Intact nuclei from G. mustelinum (AD4) were used to isolate high molecular weight DNA, which was partially cleaved with Hind III and cloned into pSMART BAC (Hind III) vectors. The BAC library consisted of 208,182 clones arrayed in 542 384-microtiter plates, with an average insert size of 121.72 kb ranging from 100 to 150 kb. About 2% of the clones did not contain inserts. Based on an estimated genome size of 2372 Mb for G. mustelinum, the BAC library was estimated to have a total coverage of 10.50 × genome equivalents. The high capacity library of G. mustelinum will serve as a giant gene resource for map-based cloning of quantitative trait loci or genes associated with important agronomic traits or resistance to Verticillium wilt, physical mapping and comparative genome analysis.
URI
http://hdl.handle.net/10342/8417
Date
2018-05-17
Citation:
APA:
Liu, Yuling, & Zhang, Baohong, & Wen, Xinpeng, & Zhang, Shulin, & Wei, Yangyang, & Lu, Quanwei, & Liu, Zhen, & Wang, Kunbo, & Liu, Fang, & Peng, Renhai. (May 2018). Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum. PLoS ONE, (13:5), p.e0196847. Retrieved from http://hdl.handle.net/10342/8417

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MLA:
Liu, Yuling, and Zhang, Baohong, and Wen, Xinpeng, and Zhang, Shulin, and Wei, Yangyang, and Lu, Quanwei, and Liu, Zhen, and Wang, Kunbo, and Liu, Fang, and Peng, Renhai. "Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum". PLoS ONE. 13:5. (e0196847.), May 2018. May 15, 2021. http://hdl.handle.net/10342/8417.
Chicago:
Liu, Yuling and Zhang, Baohong and Wen, Xinpeng and Zhang, Shulin and Wei, Yangyang and Lu, Quanwei and Liu, Zhen and Wang, Kunbo and Liu, Fang and Peng, Renhai, "Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum," PLoS ONE 13, no. 5 (May 2018), http://hdl.handle.net/10342/8417 (accessed May 15, 2021).
AMA:
Liu, Yuling, Zhang, Baohong, Wen, Xinpeng, Zhang, Shulin, Wei, Yangyang, Lu, Quanwei, Liu, Zhen, Wang, Kunbo, Liu, Fang, Peng, Renhai. Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum. PLoS ONE. May 2018; 13(5) e0196847. http://hdl.handle.net/10342/8417. Accessed May 15, 2021.
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