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    Cryo-electron tomography of periplasmic flagella in Borrelia burgdorferi reveals a distinct cytoplasmic ATPase complex.

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    Author
    Qin, Zhuan; Tu, Jiagang; Lin, Tao; Norris, Steven J.; Li, Chunhao; Motaleb, Md A.
    Abstract
    Periplasmic flagella are essential for the distinct morphology and motility of spirochetes. A flagella-specific type III secretion system (fT3SS) composed of a membrane-bound export apparatus and a cytosolic ATPase complex is responsible for the assembly of the periplasmic flagella. Here, we deployed cryo-electron tomography (cryo-ET) to visualize the fT3SS machine in the Lyme disease spirochete Borrelia burgdorferi. We show, for the first time, that the cytosolic ATPase complex is attached to the flagellar C-ring through multiple spokes to form the “spoke and hub” structure in B. burgdorferi. This structure not only strengthens structural rigidity of the round-shaped C-ring but also appears to rotate with the C-ring. Our studies provide structural insights into the unique mechanisms underlying assembly and rotation of the periplasmic flagella and may provide the basis for the development of novel therapeutic strategies against several pathogenic spirochetes.
    URI
    http://hdl.handle.net/10342/8433
    Date
    2018-11-09
    Citation:
    APA:
    Qin, Zhuan, & Tu, Jiagang, & Lin, Tao, & Norris, Steven J., & Li, Chunhao, & Motaleb, Md A.. (November 2018). Cryo-electron tomography of periplasmic flagella in Borrelia burgdorferi reveals a distinct cytoplasmic ATPase complex.. PLoS Biology, (16:11), p.e3000050. Retrieved from http://hdl.handle.net/10342/8433

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Qin, Zhuan, and Tu, Jiagang, and Lin, Tao, and Norris, Steven J., and Li, Chunhao, and Motaleb, Md A.. "Cryo-electron tomography of periplasmic flagella in Borrelia burgdorferi reveals a distinct cytoplasmic ATPase complex.". PLoS Biology. 16:11. (e3000050.), November 2018. August 10, 2022. http://hdl.handle.net/10342/8433.
    Chicago:
    Qin, Zhuan and Tu, Jiagang and Lin, Tao and Norris, Steven J. and Li, Chunhao and Motaleb, Md A., "Cryo-electron tomography of periplasmic flagella in Borrelia burgdorferi reveals a distinct cytoplasmic ATPase complex.," PLoS Biology 16, no. 11 (November 2018), http://hdl.handle.net/10342/8433 (accessed August 10, 2022).
    AMA:
    Qin, Zhuan, Tu, Jiagang, Lin, Tao, Norris, Steven J., Li, Chunhao, Motaleb, Md A.. Cryo-electron tomography of periplasmic flagella in Borrelia burgdorferi reveals a distinct cytoplasmic ATPase complex.. PLoS Biology. November 2018; 16(11) e3000050. http://hdl.handle.net/10342/8433. Accessed August 10, 2022.
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