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    Deletion of BmoR affects the expression of genes related to thiol/disulfide balance in Bacteroides fragilis

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    Author
    Teixeira, Felipe L.; Pauer, Heidi; Costa, Scarlathe B.; Smith, C. Jeffrey; Domingues, Regina M. C. P.; Rocha, Edson R.; Lobo, Leandro A.
    Abstract
    Bacteroides fragilis, an opportunistic pathogen and commensal bacterium in the gut, is one the most aerotolerant species among strict anaerobes. However, the mechanisms that control gene regulation in response to oxidative stress are not completely understood. In this study, we show that the MarR type regulator, BmoR, regulates the expression of genes involved in the homeostasis of intracellular redox state. Transcriptome analysis showed that absence of BmoR leads to altered expression in total of 167 genes. Sixteen of these genes had a 2-fold or greater change in their expression. Most of these genes are related to LPS biosynthesis and carbohydrates metabolism, but there was a signifcant increase in the expression of genes related to the redox balance inside the cell. A pyridine nucleotide-disulfde oxidoreductase located directly upstream of bmoR was shown to be repressed by direct binding of BmoR to the promoter region. The expression of two other genes, coding for a thiosulphate:quinoneoxidoreductase and a thioredoxin, are indirectly afected by bmoR mutation during oxygen exposure. Phenotypic assays showed that BmoR is important to maintain the thiol/disulfde balance in the cell, confrming its relevance to B. fragilis response to oxidative stress.
    URI
    http://hdl.handle.net/10342/8495
    Date
    2018-09-26
    Citation:
    APA:
    Teixeira, Felipe L., & Pauer, Heidi, & Costa, Scarlathe B., & Smith, C. Jeffrey, & Domingues, Regina M. C. P., & Rocha, Edson R., & Lobo, Leandro A.. (September 2018). Deletion of BmoR affects the expression of genes related to thiol/disulfide balance in Bacteroides fragilis. Scientific Reports, (14405. Retrieved from http://hdl.handle.net/10342/8495

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    MLA:
    Teixeira, Felipe L., and Pauer, Heidi, and Costa, Scarlathe B., and Smith, C. Jeffrey, and Domingues, Regina M. C. P., and Rocha, Edson R., and Lobo, Leandro A.. "Deletion of BmoR affects the expression of genes related to thiol/disulfide balance in Bacteroides fragilis". Scientific Reports. . (14405.), September 2018. April 19, 2021. http://hdl.handle.net/10342/8495.
    Chicago:
    Teixeira, Felipe L. and Pauer, Heidi and Costa, Scarlathe B. and Smith, C. Jeffrey and Domingues, Regina M. C. P. and Rocha, Edson R. and Lobo, Leandro A., "Deletion of BmoR affects the expression of genes related to thiol/disulfide balance in Bacteroides fragilis," Scientific Reports 8, no. (September 2018), http://hdl.handle.net/10342/8495 (accessed April 19, 2021).
    AMA:
    Teixeira, Felipe L., Pauer, Heidi, Costa, Scarlathe B., Smith, C. Jeffrey, Domingues, Regina M. C. P., Rocha, Edson R., Lobo, Leandro A.. Deletion of BmoR affects the expression of genes related to thiol/disulfide balance in Bacteroides fragilis. Scientific Reports. September 2018; 8() 14405. http://hdl.handle.net/10342/8495. Accessed April 19, 2021.
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