Computational identification of microRNAs associated to both epithelial to mesenchymal transition and NGAL/MMP-9 pathways in bladder cancer
Author
Falzone, Luca; Candido, Saverio; Salemi, Rossella; Basile, Maria S.; Scalisi, Aurora; McCubrey, James A.; Torino, Francesco; Signorelli, Salvatore S.; Montella, Maurizio; Libra, Massimo
Abstract
Bladder cancer is one of the leading cancer of the urinary tract. It is often diagnosed at advanced stage of the disease. To date, no specific and effective early detection biomarkers are available. Cancer development and progression are associated with the involvement of both epithelial-mesenchymal transition (EMT) and tumor microenvironment of which NGAL/MMP-9 complex represents the main player in bladder cancer. It is known that change in microRNAs (miRNAs) expression may result in gene modulation. Therefore, the identification of specific miRNAs associated with EMT pathway and NGAL/MMP-9 complex may be useful to detect the development of bladder cancer at early stages.
On this ground, the expression levels of miRNAs in public available datasets of bladder cancer containing data of non-coding RNA profiling was evaluated. This analysis revealed a group of 16 miRNAs differentially expressed between bladder cancer patients and related healthy controls. By miRNA prediction tool (mirDIP), the relationship between the identified miRNAs and the EMT genes was established. Using the DIANA-mirPath (v.2) software, miRNAs, able to modulate the expression of NGAL and MMP-9 genes, were recognized.
The results of this study provide evidence that the downregulated hsa-miR-145-5p and hsa-miR-214-3p may modulate the expression of both EMT and NGAL/MMP-9 pathways. Therefore, further validation analyses may confirm the usefulness of these selected miRNAs for predicting the development of bladder cancer at the early stage of the disease.
Date
2016-11-08
Citation:
APA:
Falzone, Luca, & Candido, Saverio, & Salemi, Rossella, & Basile, Maria S., & Scalisi, Aurora, & McCubrey, James A., & Torino, Francesco, & Signorelli, Salvatore S., & Montella, Maurizio, & Libra, Massimo. (November 2016).
Computational identification of microRNAs associated to both epithelial to mesenchymal transition and NGAL/MMP-9 pathways in bladder cancer.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/8497
MLA:
Falzone, Luca, and Candido, Saverio, and Salemi, Rossella, and Basile, Maria S., and Scalisi, Aurora, and McCubrey, James A., and Torino, Francesco, and Signorelli, Salvatore S., and Montella, Maurizio, and Libra, Massimo.
"Computational identification of microRNAs associated to both epithelial to mesenchymal transition and NGAL/MMP-9 pathways in bladder cancer". .
. (),
November 2016.
October 01, 2023.
http://hdl.handle.net/10342/8497.
Chicago:
Falzone, Luca and Candido, Saverio and Salemi, Rossella and Basile, Maria S. and Scalisi, Aurora and McCubrey, James A. and Torino, Francesco and Signorelli, Salvatore S. and Montella, Maurizio and Libra, Massimo,
"Computational identification of microRNAs associated to both epithelial to mesenchymal transition and NGAL/MMP-9 pathways in bladder cancer," , no.
(November 2016),
http://hdl.handle.net/10342/8497 (accessed
October 01, 2023).
AMA:
Falzone, Luca, Candido, Saverio, Salemi, Rossella, Basile, Maria S., Scalisi, Aurora, McCubrey, James A., Torino, Francesco, Signorelli, Salvatore S., Montella, Maurizio, Libra, Massimo.
Computational identification of microRNAs associated to both epithelial to mesenchymal transition and NGAL/MMP-9 pathways in bladder cancer. .
November 2016;
():
.
http://hdl.handle.net/10342/8497. Accessed
October 01, 2023.
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