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    Characterizations of Cross-Bridges in the Presence of Saturating Concentrations of MgAMP-PNP in Rabbit Permeabilized Psoas Muscle

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    Author
    Frisbie, S.M.; Xu, S.; Chalovich, Joseph; Yu, L.C.
    Abstract
    Several earlier studies have led to different conclusions about the complex of myosin with MgAMP-PNP. It has been suggested that subfragment 1 of myosin (S1)-MgAMP-PNP forms an S1-MgADP-like state, an intermediate between the myosin S1-MgATP and myosin S1-MgADP states or a mixture of cross-bridge states. We suggest that the different states observed result from the failure to saturate S1 with MgAMP-PNP. At saturating MgAMP-PNP, the interaction of myosin S1 with actin is very similar to that which occurs in the presence of MgATP. 1) At 1°C and 170 mM ionic strength the equatorial x-ray diffraction intensity ratio I11/I10 decreased with an increasing MgAMP-PNP concentration and leveled off by ∼20 mM MgAMP-PNP. The resulting ratio was the same for MgATP-relaxed fibers. 2) The two dimensional x-ray diffraction patterns from MgATP-relaxed and MgAMP-PNP-relaxed bundles are similar. 3) The affinity of S1-MgAMP-PNP for the actin-tropomyosin-troponin complex in solution in the absence of free calcium is comparable with that of S1-MgATP. 4) In the presence of calcium, I11/I10 decreased toward the relaxed value with increasing MgAMP-PNP, signifying that the affinity between cross-bridge and actin is weakened by MgAMP-PNP. 5) The degree to which the equatorial intensity ratio decreases as the ionic strength increases is similar in MgAMP-PNP and MgATP. Therefore, results from both fiber and solution studies suggest that MgAMP-PNP acts as a non hydrolyzable MgATP analogue for myosin.
    URI
    http://hdl.handle.net/10342/8876
    Subject
    AMP-PNP5′-adenylylimido-diphosphate
    Date
    1998-06
    Citation:
    APA:
    Frisbie, S.M., & Xu, S., & Chalovich, Joseph, & Yu, L.C.. (June 1998). Characterizations of Cross-Bridges in the Presence of Saturating Concentrations of MgAMP-PNP in Rabbit Permeabilized Psoas Muscle. , (), - . Retrieved from http://hdl.handle.net/10342/8876

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Frisbie, S.M., and Xu, S., and Chalovich, Joseph, and Yu, L.C.. "Characterizations of Cross-Bridges in the Presence of Saturating Concentrations of MgAMP-PNP in Rabbit Permeabilized Psoas Muscle". . . (), June 1998. September 27, 2023. http://hdl.handle.net/10342/8876.
    Chicago:
    Frisbie, S.M. and Xu, S. and Chalovich, Joseph and Yu, L.C., "Characterizations of Cross-Bridges in the Presence of Saturating Concentrations of MgAMP-PNP in Rabbit Permeabilized Psoas Muscle," , no. (June 1998), http://hdl.handle.net/10342/8876 (accessed September 27, 2023).
    AMA:
    Frisbie, S.M., Xu, S., Chalovich, Joseph, Yu, L.C.. Characterizations of Cross-Bridges in the Presence of Saturating Concentrations of MgAMP-PNP in Rabbit Permeabilized Psoas Muscle. . June 1998; (): . http://hdl.handle.net/10342/8876. Accessed September 27, 2023.
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