Enhanced Mitochondrial Superoxide Scavenging Does Not Improve Muscle Insulin Action in the High Fat-Fed Mouse
dc.contributor.author | Lark, Daniel S. | |
dc.contributor.author | Kang, Li | |
dc.contributor.author | Lustig, Mary E. | |
dc.contributor.author | Bonner, Jeffrey S. | |
dc.contributor.author | James, Freyja D. | |
dc.contributor.author | Neufer, Darrell | |
dc.contributor.author | Wasserman, David H. | |
dc.date.accessioned | 2020-04-13T17:20:56Z | |
dc.date.available | 2020-04-13T17:20:56Z | |
dc.date.issued | 2015-05 | |
dc.description.abstract | Improving mitochondrial oxidant scavenging may be a viable strategy for the treatment of insulin resistance and diabetes. Mice overexpressing the mitochondrial matrix isoform of superoxide dismutase (sod2tg mice) and/or transgenically expressing catalase within the mitochondrial matrix (mcattg mice) have increased scavenging of O2˙ˉ and H2O2, respectively. Furthermore, muscle insulin action is partially preserved in high fat (HF)-fed mcattg mice. The goal of the current study was to test the hypothesis that increased O2˙ˉ scavenging alone or in combination with increased H2O2 scavenging (mtAO mice) enhances in vivo muscle insulin action in the HF-fed mouse. Insulin action was examined in conscious, unrestrained and unstressed wild type (WT), sod2tg, mcattg and mtAO mice using hyperinsulinemic-euglycemic clamps (insulin clamps) combined with radioactive glucose tracers following sixteen weeks of normal chow or HF (60% calories from fat) feeding. Glucose infusion rates, whole body glucose disappearance, and muscle glucose uptake during the insulin clamp were similar in chow- and HF-fed WT and sod2tg mice. Consistent with our previous work, HF-fed mcattg mice had improved muscle insulin action, however, an additive effect was not seen in mtAO mice. Insulin-stimulated Akt phosphorylation in muscle from clamped mice was consistent with glucose flux measurements. These results demonstrate that increased O2˙ˉ scavenging does not improve muscle insulin action in the HF-fed mouse alone or when coupled to increased H2O2 scavenging. | en_US |
dc.identifier.doi | 10.1371/journal.pone.0126732 | |
dc.identifier.uri | http://hdl.handle.net/10342/8108 | |
dc.title | Enhanced Mitochondrial Superoxide Scavenging Does Not Improve Muscle Insulin Action in the High Fat-Fed Mouse | en_US |
dc.type | Article | en_US |
ecu.journal.issue | 5 | en_US |
ecu.journal.name | PLOS One | en_US |
ecu.journal.volume | 10 | en_US |
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