Isolation and characterization of Bordetella bronchiseptica mutants deficient in siderophore activity.

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dc.contributor.authorArmstrong, Sandra K.en_US
dc.contributor.authorClements, Mark O.en_US
dc.date.accessioned2011-04-28T19:30:45Zen_US
dc.date.accessioned2011-05-17T01:40:11Z
dc.date.available2011-04-28T19:30:45Zen_US
dc.date.available2011-05-17T01:40:11Z
dc.date.issued1993-02en_US
dc.description.abstractIron acquisition by the gram-negative pathogens Bordetella bronchiseptica and Bordetella pertussis is thought to occur by hydroxamate siderophore-mediated transport as well as an apparently siderophore-independent process by which host transferrins bind to bacterial surface receptors. We constructed B. bronchiseptica mutants deficient in siderophore activity by insertional mutagenesis with miniTn5/LacZl. The mutants could be placed into four distinct complementation groups, as determined from cross-feeding assays which demonstrated restored siderophore synthesis. Mutants deficient in siderophore activity were BRM1, BRM6, and BRM9, exhibiting approximately 36 to 41% of wild-type siderophore levels, and BRM3 and BRM8, which appeared to produce very little or no detectable siderophore. Mutant BRM4 was found to be a leucine auxotroph, while mutants BRM2 and BRM7 could synthesize siderophore only in low-iron medium which was supplemented with various amino acids. Evaluation of all transcriptional fusions revealed an apparent lack of iron-regulated lacZ expression. Genomic regions flanking the transposable element in the siderophore mutants were homologous with B. pertussis chromosomal DNA, while bioassays suggested siderophore cross-feeding between B. pertussis and B. bronchiseptica. These results indicate probable similarity between the siderophore biosynthetic and transport systems of the two species. Originally published Journal of Bacteriology, Vol. 175, No. 4, Feb 1993en_US
dc.identifier.citationJournal of Bacteriology; 175:4 p. 1144-1152en_US
dc.identifier.pmidPMC193031en_US
dc.identifier.urihttp://hdl.handle.net/10342/3437en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://jb.asm.org/archive/1993.dtlen_US
dc.rightsAuthor notified of opt-out rights by Cammie Jennings prior to upload of this article.en_US
dc.subjectBordetella bronchisepticaen_US
dc.subjectSiderophore activityen_US
dc.subjectIron acquisitionen_US
dc.titleIsolation and characterization of Bordetella bronchiseptica mutants deficient in siderophore activity.en_US
dc.typeArticleen_US

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