Quinolone signaling in the cell-to-cell communication system of Pseudomonas aeruginosa

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dc.contributor.authorPesci, Everett C.en_US
dc.contributor.authorMilbank, Jared B. J.en_US
dc.contributor.authorPearson, James P.en_US
dc.contributor.authorMcKnight, Susan L.en_US
dc.contributor.authorKende, Andrew S.en_US
dc.contributor.authorGreenberg, E. Peteren_US
dc.contributor.authorIglewski, Barbara H.en_US
dc.date.accessioned2011-01-21T20:52:01Zen_US
dc.date.accessioned2011-05-17T01:39:59Z
dc.date.available2011-01-21T20:52:01Zen_US
dc.date.available2011-05-17T01:39:59Z
dc.date.issued1999-09-28en_US
dc.description.abstractNumerous species of bacteria use an elegant regulatory mechanism known as quorum sensing to control the expression of specific genes in a cell-density dependent manner. In Gram-negative bacteria, quorum sensing systems function through a cell-to-cell signal molecule (autoinducer) that consists of a homoserine lactone with a fatty acid side chain. Such is the case in the opportunistic human pathogen Pseudomonas aeruginosa, which contains two quorum sensing systems (las and rhl) that operate via the autoinducers, N-(3-oxododecanoyl)-L-homoserine lactone and N-butyryl-Lhomoserine lactone. The study of these signal molecules has shown that they bind to and activate transcriptional activator proteins that specifically induce numerous P. aeruginosa virulence genes. We report here that P. aeruginosa produces another signal molecule, 2-heptyl-3-hydroxy-4-quinolone, which has been designated as the Pseudomonas quinolone signal. It was found that this unique cell-to-cell signal controlled the expression of lasB, which encodes for the major virulence factor, LasB elastase. We also show that the synthesis and bioactivity of Pseudomonas quinolone signal were mediated by the P. aeruginosa las and rhl quorum sensing systems, respectively. The demonstration that 2-heptyl-3- hydroxy-4-quinolone can function as an intercellular signal sheds light on the role of secondary metabolites and shows that P. aeruginosa cell-to-cell signaling is not restricted to acyl-homoserine lactones. Originally published Proc. Natl. Acad. Sci, Vol. 96, No. 20, Sep. 1999en_US
dc.identifier.citationProceedings of the National Academy of Sciences; 96:20 p. 11229-11234en_US
dc.identifier.pmidPMC18016en_US
dc.identifier.urihttp://hdl.handle.net/10342/3071en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://www.pnas.org/content/by/year/1999en_US
dc.subjectQuorum sensingen_US
dc.subjectHomoserine lactoneen_US
dc.subjectBacteria regulation mechanismen_US
dc.titleQuinolone signaling in the cell-to-cell communication system of Pseudomonas aeruginosaen_US
dc.typeArticleen_US

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