A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation

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dc.contributor.authorQi, Yiping
dc.contributor.authorLowder, Levi G.
dc.contributor.authorZhang, Dengwei
dc.contributor.authorBaltes, Nicholas J.
dc.contributor.authorPaul, Joseph W. III
dc.contributor.authorTang, Xu
dc.contributor.authorZheng, Xuelian
dc.contributor.authorVoytas, Daniel F.
dc.contributor.authorHsieh, Tzung-Fu
dc.contributor.authorZhang, Yong
dc.date.accessioned2015-09-17T19:52:07Z
dc.date.available2015-09-17T19:52:07Z
dc.date.issued2015
dc.description.abstractThe relative ease, speed and biological scope of CRISPR/Cas9-based reagents for genomic manipulations are revolutionizing virtually all areas of molecular biosciences, including functional genomics, genetics, applied biomedical research and agricultural biotechnology. In plant systems, however, a number of hurdles currently exist that limit this technology from reaching its full potential. For example, significant plant molecular biology expertise and effort is still required to generate functional expression constructs that allow simultaneous editing, and especially transcriptional regulation, of multiple different genomic loci or "multiplexing", which is a significant advantage of CRISPR/Cas9 versus other genome editing systems. In order to streamline and facilitate rapid and wide-scale use of CRISPR/Cas9-based technologies for plant research, we developed and implemented a comprehensive molecular toolbox for multifaceted CRISPR/Cas9 applications in plants. This toolbox provides researchers with a protocol and reagents to quickly and efficiently assemble functional CRISPR/Cas9 T-DNA constructs for monocots and dicots using Golden Gate and Gateway cloning methods. It comes with a full suite of capabilities, including multiplexed gene editing and transcriptional activation or repression of plant endogenous genes. We report the functionality and effectiveness of this toolbox in model plants such as tobacco, Arabidopsis and rice, demonstrating its utility for basic and applied plant research.en_US
dc.description.sponsorshipECU Open Access Publishing Support Fund
dc.identifier.citationPlant Physiology; 169:2 p. 971-985en_US
dc.identifier.doidx.​doi.​org/​10.​1104/​pp.​15.​00636
dc.identifier.pmid26297141en_US
dc.identifier.urihttp://hdl.handle.net/10342/5051
dc.relation.urihttp://www.plantphysiol.org/content/early/2015/08/21/pp.15.00636.abstracten_US
dc.titleA CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulationen_US
dc.typeArticleen_US
ecu.journal.issue2
ecu.journal.namePlant Physiology
ecu.journal.pages971-985
ecu.journal.volume169

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