Inhibition of Nischarin Expression Attenuates Rilmenidine-Evoked Hypotension and Phosphorylated Extracellular Signal-Regulated Kinase 1/2 Production in the Rostral Ventrolateral Medulla of Rats

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dc.contributor.authorZhang, Jianen_US
dc.contributor.authorAbdel-Rahman, Abdel A.en_US
dc.date.accessioned2011-01-28T19:46:32Zen_US
dc.date.accessioned2011-05-17T13:10:55Z
dc.date.available2011-01-28T19:46:32Zen_US
dc.date.available2011-05-17T13:10:55Z
dc.date.issued2008-01en_US
dc.description.abstractImidazoline (I1)-evoked hypotension is linked to enhanced phosphorylated extracellular signalregulated kinase (pERK)1/2 production in the rostral ventrolateral medulla (RVLM). Recent cell culture findings suggest that nischarin is a candidate for the I1 receptor. In the present study, nischarin antisense oligode-oxynucleotide (ODN) (AS1 or AS2), designed according to nischarin cDNA sequence, was administered intracisternally (i.c., 2 nmol/rat for 2 days) to knockdown central nischarin expression; control rats received the corresponding mismatched ODN (MM1 or MM2) or artificial cerebrospinal fluid (aCSF). We investigated the effects of AS1 or AS2 on nischarin expression in the RVLM, and on the hypotension and RVLM pERK1/2 production elicited by the I1-selective agonist rilmenidine (25 μ g/rat i.c.). Compared with aCSF, the mismatched ODN (MM1 or MM2) had no significant effect on RVLM nischarin expression or the cardiovascular and cellular (RVLM pERK1/2) responses elicited by rilmenidine. However, either antisense ODN substantially (>80%) reduced nischarin expression in the RVLM (AS1/MM1, 3 ± 1 versus 32 ± 2 positive cells; AS2/MM2, 4 ± 1 versus 31 ± 2 positive cells) and abrogated rilmenidine (I1)-evoked hypotension (AS1/MM1, −4.1 ± 0.9 versus −10.8 ± 1.9 mm Hg; AS2/MM2, −2.1 ± 1.1 versus −15.3 ± 2.5 mm Hg) and ERK1/2 activation in the RVLM (AS1/MM1, 10 ± 1 versus 15 ± 2 positive cells; AS2/MM2, 9 ± 1 versus 18 ± 2 positive cells). Finally, pERK1/2 generated by central I1 receptor activation is colocalized with nischarin in the RVLM neurons. This is the first evidence in vivo that nischarin plays a critical role in I1 receptor-mediated pERK1/2 production in the RVLM and the subsequent hypotension. Origianlly published Journal of Pharmacology and Experimental Therapeutics, Vol. 324, No. 1, Jan 2008en_US
dc.identifier.citationJournal of Pharmacology and Experimental Therapeutics; 324:1 p. 72-78en_US
dc.identifier.doi10.1124/jpet.107.129049
dc.identifier.pmidPMC2704025en_US
dc.identifier.urihttp://hdl.handle.net/10342/3135en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://jpet.aspetjournals.org/content/324/1/72.longen_US
dc.rightsAuthor notified of opt-out rights by Cammie Jenningsen_US
dc.subjectNischarinen_US
dc.subjectImidazoline evoked hypotensionen_US
dc.subjectSignal regulated kinaseen_US
dc.titleInhibition of Nischarin Expression Attenuates Rilmenidine-Evoked Hypotension and Phosphorylated Extracellular Signal-Regulated Kinase 1/2 Production in the Rostral Ventrolateral Medulla of Ratsen_US
dc.typeArticleen_US
ecu.journal.issue1
ecu.journal.nameJournal of Pharmacology and Experimental Therapeutics
ecu.journal.pages72-78
ecu.journal.volume324

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