Brain transcriptome sequencing and assembly of three songbird model systems for the study of social behavior

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dc.contributor.authorBalakrishnan, Christopher N.
dc.contributor.authorMukai, Motoko
dc.contributor.authorGonser, Rusty A.
dc.contributor.authorWingfield, John C.
dc.contributor.authorLondon, Sarah E.
dc.contributor.authorTuttle, Elaina M.
dc.contributor.authorClayton, David F.
dc.date.accessioned2016-06-07T17:40:01Z
dc.date.available2016-06-07T17:40:01Z
dc.date.issued2014-05
dc.description.abstractEmberizid sparrows (emberizidae) have played a prominent role in the study of avian vocal communication and social behavior. We present here brain transcriptomes for three emberizid model systems, song sparrow Melospiza melodia, white-throated sparrow Zonotrichia albicollis, and Gambel’s white-crowned sparrow Zonotrichia leucophrys gambelii. Each of the assemblies covered fully or in part, over 89% of the previously annotated protein coding genes in the zebra finch Taeniopygia guttata, with 16,846, 15,805, and 16,646 unique BLAST hits in song, white-throated and white-crowned sparrows, respectively. As in previous studies, we find tissue of origin (auditory forebrain versus hypothalamus and whole brain) as an important determinant of overall expression profile. We also demonstrate the successful isolation of RNA and RNA-sequencing from post-mortem samples from building strikes and suggest that such an approach could be useful when traditional sampling opportunities are limited. These transcriptomes will be an important resource for the study of social behavior in birds and for data driven annotation of forthcoming whole genome sequences for these and other bird species.en_US
dc.identifier.citationPeerJ; 2: p. 1-17en_US
dc.identifier.doi10.7717/peerj.396
dc.identifier.issn2167-8359
dc.identifier.pmidpmc4034602en_US
dc.identifier.urihttp://hdl.handle.net/10342/5505
dc.relation.urihttps://peerj.com/articles/396/en_US
dc.subjectAnimal Behavioren_US
dc.subjectNeuroscienceen_US
dc.subjectGenomicsen_US
dc.subjectEvolutionary Studiesen_US
dc.titleBrain transcriptome sequencing and assembly of three songbird model systems for the study of social behavioren_US
dc.typeArticleen_US
ecu.journal.namePeerJen_US
ecu.journal.pages1-17en_US
ecu.journal.volume2en_US

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