Adamts9 is necessary for ovarian development in zebrafish

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dc.access.optionRestricted Campus Access Only
dc.contributor.advisorZhu, Yong
dc.contributor.advisorCarter, Nichole J.
dc.contributor.authorRoach, Zachary Adam
dc.contributor.departmentBiology
dc.date.accessioned2021-05-08T17:27:08Z
dc.date.available2021-05-08T17:27:08Z
dc.date.created2020-05
dc.date.issued2020-05-04
dc.date.submittedMay 2020
dc.date.updated2021-04-23T19:53:55Z
dc.degree.departmentBiology
dc.degree.disciplineBiochemistry
dc.degree.grantorEast Carolina University
dc.degree.levelUndergraduate
dc.degree.nameBS
dc.description.abstractExpression of adamts9 (A disintegrin and metalloprotease with thrombospondin type-1 motif, member 9) increases dramatically in the somatic cells surrounding oocytes during ovulation in vertebrates from zebrafish to human. However, the function of Adamts9 during ovulation has not been determined due to the embryonic lethality of knockouts in mice and Drosophila. To identify the role of Adamts9 during ovulation we generated knockout (adamts9−/−) zebrafish using CRISPR/Cas9 and characterized the effects of the mutation. From 1047 fish generated by crossing adamts9+/− pairs, we found significantly fewer adult adamts9−/− fish (4%) than predicted by Mendelian ratios (25%). Of the mutants found, there was a significant male bias (82%). Only 3 female mutants were identified (7%), and they had small ovaries with few stage III and IV oocytes compared to wildtype (wt) counterparts of comparable size and age. Astoundingly, the remaining mutants (11%) did not appear to have normal testis or ovaries. Instead there was a pair of transparent, ovarian-like membranous shells that filled the abdominal cavity. Histological examination confirmed that shells were largely empty with no internal structure. Surprisingly, seminiferous tubules and various spermatocytes including mature spermatozoa were observed on the periphery of these transparent shells. No female or female like knockouts were observed to release eggs, and no ovulated oocytes were observed in histological sections. To our knowledge, this is the first report of an adamts9 global knockout model in any adult vertebrates and the first description of how gonadal sex and structure are affected- highlighting the importance of Adamts9 during gonadal development and the value of zebrafish as a model organism.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/10342/9007
dc.publisherEast Carolina University
dc.subjectAdamts9
dc.subjectMetalloproteinase
dc.subjectOvarian development
dc.subjectSexual development
dc.subjectOvulation
dc.subjectZebrafish
dc.titleAdamts9 is necessary for ovarian development in zebrafish
dc.typeHonors Thesis
dc.type.materialtext

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