Development of a Method for the Determination of Bisphenol A in Vervet Monkey Hair using LC-MS/MS and LC-UV/Vis

Abstract

No methods for estimating levels of anthropogenic disturbance on non-human primate (NHP) populations were identified within the literature. Such a method would allow for the assessment of human impact on NHP populations. Levels of bisphenol A (BPA) in hair samples was the proposed metric for such a method. Human hair samples were used to validate a procedure found in the literature.1 A methanolic extraction was performed on human hair and the resulting samples were analyzed using liquid chromatography tandem mass spectrometry (LC-MS/MS). The two extracted samples contained 110.1 and 37.1 pg BPA mg-1 hair. Extracted, spiked samples yielded 913.9 and 885.7 pg BPA mg-1 hair. Control samples of standard yielded 200 times lower than the expected concentration. A control alkaline extraction was attempted but not found to be suitable. Standards over the expected concertation range of prepared extractions were run on a liquid chromatography – ultraviolet visible spectrophotometry (LC-UV/Vis) system were used to generate a standard curve with an R2 value of 0.9993.