Kinetics of Binding of Caldesmon to Actin

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dc.contributor.authorChalovich, Josephen_US
dc.contributor.authorChen, Yi-Deren_US
dc.contributor.authorDudek, Ronald W.en_US
dc.contributor.authorHai, Luoen_US
dc.date.accessioned2010-11-08T19:07:55Zen_US
dc.date.accessioned2011-05-17T01:27:03Z
dc.date.available2010-11-08T19:07:55Zen_US
dc.date.available2011-05-17T01:27:03Z
dc.date.issued1995-04-28en_US
dc.description.abstractThe time course of interaction of caldesmon with actin may be monitored by fluorescence changes that occur upon the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or to acrylodan actin. The concentration dependence of the observed rate of caldesmon-actin binding was analyzed to a first approximation as a single-step reaction using a Monte Carlo simulation. The derived association and dissociation rates were 107 M-1 s-1 and 18.2 s-1, respectively. Smooth muscle tropomyosin enhances the binding of caldesmon to actin, and this was found to be due to a reduction in the rate of dissociation to 6.3 s -1. There is no evidence from this study for a different mechanism of binding in the presence of tropomyosin. The fluorescence changes that occurred with the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or actin-tropomyosin were reversed by the addition of myosin subfragment 1 as predicted by a competitive binding mechanism. Originally published in the Journal of Biological Chemistry, Vol. 270, No. 17, 1995.en_US
dc.identifier.citationJournal of Biological Chemistry; 270:17 p. 9911-9916en_US
dc.identifier.doi10.1074/jbc.270.17.9911
dc.identifier.pmidPMC1262633en_US
dc.identifier.urihttp://hdl.handle.net/10342/2994en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://www.jbc.org/search?author1=&fulltext=&pubdate_year=1995&volume=270&firstpage=9911&submit=yesen_US
dc.rightsPermission granted by author to Kent Nixon Myers to upload this article on 10/03/2010.en_US
dc.subjectCaldesmon interactionsen_US
dc.subjectFlourescence changes in labeled moleculesen_US
dc.subjectActin bindingen_US
dc.titleKinetics of Binding of Caldesmon to Actinen_US
dc.typeArticleen_US

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