Epitope tags are not created equal: disruption of cellular function of a translation factor by a short viral tag

dc.access.option	Restricted Campus Access Only
dc.contributor.advisor	Keiper, Brett D
dc.contributor.author	Barker, Brooke K
dc.contributor.department	Biochemistry and Molecular Bio
dc.date.accessioned	2025-02-20T20:39:38Z
dc.date.created	2024-12
dc.date.issued	2024-12-04
dc.date.submitted	December 2024
dc.date.updated	2025-02-06T14:50:40Z
dc.degree.department	Biochemistry and Molecular Bio
dc.degree.discipline	Biology
dc.degree.grantor	East Carolina University
dc.degree.level	Undergraduate
dc.degree.name	BS
dc.description.abstract	Cellular identity and fate are determined by the proteins synthesized. Initiation of mRNA translation requires an important translation factor eIF4G (ifg-1 in C elegans). Embryos use mRNA translation for spatial and temporal regulation of protein synthesis. Using CRISPR engineering, we added in-frame epitope and fluorescent tags (V5, Myc, Flag, GFP and mCherry) to IFG-1. Tagged forms containing the V5 epitope caused embryonic arrest. Internal disruption of the V5 tag restored viability at 25°C. This study demonstrates that the molecular nature of a small epitope tag is sufficient to disrupt C. elegans embryogenesis.
dc.embargo.lift	2025-12-01
dc.embargo.terms	2025-12-01
dc.format.mimetype	application/pdf
dc.identifier.uri	http://hdl.handle.net/10342/13939
dc.subject	embryogenesis, mRNA translation, protein tags
dc.title	Epitope tags are not created equal: disruption of cellular function of a translation factor by a short viral tag
dc.type	Honors Thesis
dc.type.material	text

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