Structural insights into flagellar stator– rotor interactions

Simple item page
dc.contributor.authorChang, Yunjie
dc.contributor.authorMoon, Ki Hwan
dc.contributor.authorZhao, Xiaowei
dc.contributor.authorNorris, Steven J
dc.contributor.authorMotaleb, MD A
dc.contributor.authorLiu, Jun
dc.date.accessioned2020-04-22T16:23:01Z
dc.date.available2020-04-22T16:23:01Z
dc.date.issued2019-07-17
dc.description.abstractThe bacterial flagellar motor is a molecular machine that can rotate the flagellar filament at high speed. The rotation is generated by the stator–rotor interaction, coupled with an ion flux through the torque-generating stator. Here we employed cryo-electron tomography to visualize the intact flagellar motor in the Lyme disease spirochete, Borrelia burgdorferi. By analyzing the motor structures of wild-type and stator-deletion mutants, we not only localized the stator complex in situ, but also revealed the stator–rotor interaction at an unprecedented detail. Importantly, the stator–rotor interaction induces a conformational change in the flagella C-ring. Given our observation that a non-motile mutant, in which proton flux is blocked, cannot generate the similar conformational change, we propose that the proton-driven torque is responsible for the conformational change required for flagellar rotation.en_US
dc.identifier.doi10.7554/eLife.48979
dc.identifier.urihttp://hdl.handle.net/10342/8326
dc.titleStructural insights into flagellar stator– rotor interactionsen_US
dc.typeArticleen_US
ecu.journal.nameeLifeen_US
ecu.journal.pagese48979en_US
ecu.journal.volume8en_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
elife-48979-v3.pdf
Size:
6.23 MB
Format:
Adobe Portable Document Format
Description:
Download

Collections

Open Access