Repository logo
 

The pseudorabies virus UL28 protein enters the nucleus after coexpression with the herpes simplex virus UL15 protein

dc.contributor.authorKoslowski, Kim M.en_US
dc.contributor.authorShaver, Patti R.en_US
dc.contributor.authorWang, Xiao-Yangen_US
dc.contributor.authorTenney, Daniel J.en_US
dc.contributor.authorPederson, Nels E.en_US
dc.date.accessioned2011-01-06T21:54:15Zen_US
dc.date.accessioned2011-05-17T01:40:00Z
dc.date.available2011-01-06T21:54:15Zen_US
dc.date.available2011-05-17T01:40:00Z
dc.date.issued1997-12en_US
dc.description.abstractHerpesvirus DNA is packaged into capsids in the nuclei of infected cells in a process requiring at least six viral proteins. Of the proteins required for encapsidation of viral DNA, UL15 and UL28 are the most conserved among herpes simplex virus type 1 (HSV), varicella-zoster virus, and equine herpesvirus 1. The subcellular distribution of the pseudorabies virus (PRV) UL28 protein was examined by in situ immunofluorescence. UL28 was present in the nuclei of infected cells; however, UL28 was limited to the cytoplasm in the absence of other viral proteins. When cells expressing variant forms of UL28 were infected with a PRV UL28-null mutant, UL28 entered the nucleus, provided the carboxyl-terminal 155 amino acids were present. Additionally, PRV UL28 entered the nucleus in cells infected with HSV. Two HSV packaging proteins were tested for the ability to affect the subcellular distribution of UL28. Coexpression of HSV UL15 enabled PRV UL28 to enter the nucleus in a manner that required the carboxyl-terminal 155 amino acids of UL28. Coexpression of HSV UL25 did not affect the distribution of UL28. We propose that an interaction between UL15 and UL28 facilitates the transport of a UL15-UL28 complex to the infected-cell nucleus. Originally published Journal of Virology 1997 Vol. 71, No. 12.en_US
dc.identifier.citationJournal of Virology; 71:12 p. 9118-9123en_US
dc.identifier.pmidPMC230212en_US
dc.identifier.urihttp://hdl.handle.net/10342/3035en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://jvi.asm.org/content/vol71/issue12/index.dtlen_US
dc.subjectHerpes virusen_US
dc.subjectViral Proteinsen_US
dc.subjectViral DNAen_US
dc.subjectPseudorabies virusen_US
dc.titleThe pseudorabies virus UL28 protein enters the nucleus after coexpression with the herpes simplex virus UL15 proteinen_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
PseudorabiesCoexpressHerpesSimplexKoslowskietal.pdf
Size:
399.29 KB
Format:
Adobe Portable Document Format