The nucleotide sequence of the Pseudomonas aeruginosa pyrE-crc-rph region and the purification of the crc gene product.

Simple item page
dc.contributor.authorMacGregor, Carolyn H.en_US
dc.contributor.authorArora, Shiwani K.en_US
dc.contributor.authorHager, Paul W.en_US
dc.contributor.authorDail, Mary Bethen_US
dc.contributor.authorPhibbs, Paul V. Jr.en_US
dc.date.accessioned2011-04-15T16:44:32Zen_US
dc.date.accessioned2011-05-17T01:40:05Z
dc.date.available2011-04-15T16:44:32Zen_US
dc.date.available2011-05-17T01:40:05Z
dc.date.issued1996-10en_US
dc.description.abstractThe gene (crc) responsible for catabolite repression control in Pseudomonas aeruginosa has been cloned and sequenced. Flanking the crc gene are genes encoding orotate phosphoribosyl transferase (pyrE) and RNase PH (rph). New crc mutants were constructed by disruption of the wild-type crc gene. The crc gene encodes an open reading frame of 259 amino acids with homology to the apurinic/apyrimidinic endonuclease family of DNA repair enzymes. However, crc mutants do not have a DNA repair phenotype, nor can the crc gene complement Escherichia coli DNA repair-deficient strains. The crc gene product was overexpressed in both P. aeruginosa and in E. coli, and the Crc protein was purified from both. The purified Crc proteins show neither apurinic/ apyrimidinic endonuclease nor exonuclease activity. Antibody to the purified Crc protein reacted with proteins of similar size in crude extracts from Pseudomonas putida and Pseudomonas fluorescens, suggesting a common mechanism of catabolite repression in these three species. Originally published Journal of Bacteriology, Vol. 178, No. 19, Oct 1996en_US
dc.identifier.citationJournal of Bacteriology; 178:19 p. 5627-5635en_US
dc.identifier.pmidPMC178400en_US
dc.identifier.urihttp://hdl.handle.net/10342/3346en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://jb.asm.org/archive/1996.dtlen_US
dc.rightsAuthor notified of opt-out rights by Cammie Jennings prior to upload of this article.en_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectCrc geneen_US
dc.subjectCatabolite repressionen_US
dc.titleThe nucleotide sequence of the Pseudomonas aeruginosa pyrE-crc-rph region and the purification of the crc gene product.en_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Nucleotide sequence Pseudomonas aeruginosa.pdf
Size:
757.06 KB
Format:
Adobe Portable Document Format
Download

Collections

Microbiology and Immunology