American Ginseng Modifies 137Cs-Induced DNA Damage and Oxidative Stress in Human Lymphocytes

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dc.contributor.authorLee, Tung-Kwangen_US
dc.contributor.authorO'Brien, Kevin F.en_US
dc.contributor.authorWang, Weidongen_US
dc.contributor.authorSheng, Chaoen_US
dc.contributor.authorWang, Taoen_US
dc.contributor.authorJohnke, Roberta M.en_US
dc.contributor.authorAllison, Ron R.en_US
dc.date.accessioned2011-01-06T21:55:55Zen_US
dc.date.accessioned2011-05-17T00:33:29Z
dc.date.available2011-01-06T21:55:55Zen_US
dc.date.available2011-05-17T00:33:29Z
dc.date.issued2009-01-01en_US
dc.description.abstractThe multifold bioactive medicinal properties of ginseng have been closely linked to its antioxidative ability, which is related to its ginsenoside content. Since the key mechanism of radiation-induced cell death and tissue damage is the generation of reactive oxygen species (ROS) that attack cellular DNA, this study focuses on the impact of a standardized North American ginseng extract (NAGE) on 137Cs-induced oxidative stress in human peripheral lymphocytes (PBL) obtained from 10 healthy individuals (6M/4F), 42.7 ± 4.6 years of age. At two different time points (0 h and 24 h before irradiation), we applied NAGE (250 - 1000 µg ml-1) to mononuclear cell cultures for cytokinesisblock micronuclei (MN) assay and determination of the state of oxidative stress in PBL. We found that at both time points, NAGE significantly reduced the MN yields in PBL after irradiation (1 and 2 Gy) in a concentration-dependent manner (P<0.001). Compared with radiation alone, the maximum reduction rate of MN yield were 51.1% and 49.1% after 1 Gy and 2 Gy exposures, respectively. We also found that before irradiation the presence of NAGE in the culture medium resulted in a significant increased intracellular total antioxidant capacity (TAC) in PBL. At both time points, the increment of 137Cs-induced MN yields in PBL was positively correlated with the increment of intracellular ROS production (R = 0.6 - 0.7, P = 0.002), but negatively correlated with the reduction of TAC levels (R = -0.4 - 0.5, P = 0.02 - 0.004). However, the presence of NAGE in the culture medium significantly increased the TAC levels, while concomitantly decreasing both ROS production and MN yields in PBL (P<0.001). Our findings that NAGE is effective in protecting human PBL against radiation-induced oxidative stress should encourage further in vivo study of dietary supplementation with NAGE as an effective natural radiation countermeasure. Originally published Open Nuclear Medicine Journal Vol 1 No. 1, 2009.en_US
dc.identifier.citationOpen Nuclear Medicine Journal; 1:1 p. 1-8en_US
dc.identifier.doi10.2174/1876388X00901010001
dc.identifier.pmidPMC2782928en_US
dc.identifier.urihttp://hdl.handle.net/10342/3037en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://benthamscience.com/open/openaccess.php?tonmedj/article/V001/1TONMEDJ.htmen_US
dc.subjectDNA damageen_US
dc.subjectOxidative stressen_US
dc.subjectGinsengen_US
dc.titleAmerican Ginseng Modifies 137Cs-Induced DNA Damage and Oxidative Stress in Human Lymphocytesen_US
dc.typeArticleen_US
ecu.journal.issue1
ecu.journal.nameOpen Nuclear Medicine Journal
ecu.journal.pages1-8
ecu.journal.volume1

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