Calponin interaction with alpha-actinin-actin: evidence for a structural role for calponin.

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dc.contributor.authorLeinweber, Barbaraen_US
dc.contributor.authorTang, Jay X.en_US
dc.contributor.authorStafford, Walter F.en_US
dc.contributor.authorChalovich, Josephen_US
dc.date.accessioned2011-04-15T16:36:56Zen_US
dc.date.accessioned2011-05-17T01:27:02Z
dc.date.available2011-04-15T16:36:56Zen_US
dc.date.available2011-05-17T01:27:02Z
dc.date.issued1999-12en_US
dc.description.abstractThe purpose of this study was to address the paradox of calponin localization with a-actinin and filamin, two proteins with tandem calponin homology (CH) domains, by determining the effect of these proteins on the binding of calponin to actin. The results show that actin can accommodate near-saturating concentrations of either calponin and a-actinin or calponin and filamin with little change or no change in ligand affinity. Little direct interaction occurred between a-actinin and calponin in the absence of actin, so this effect is not likely to explain the co-distribution of these proteins. Calponin, like a-actinin, induced elastic gel formation when added to actin. When a-actinin was added to newly formed calponin/actin gels, no change was seen in the mechanical properties of the gel compared to calponin and actin alone. However, when calponin was added to newly formed a-actinin/actin gels, the resulting gel was much stronger than the gels formed by either ligand alone. Furthermore, gels formed by the addition of calponin to a-actinin/actin exhibited a phenomenon known as strain hardening, a characteristic of mechanically resilient gels. These results add weight to the concept that one of the functions of calponin is to stabilize the actin cytoskeleton. Originally published Biophysical Journal, Vol. 77, No. 6, Dec 1999en_US
dc.identifier.citationBiophysical Journal; 77:6 p. 3208-3217en_US
dc.identifier.doi10.1016/S0006-3495(99)77151-1
dc.identifier.pmidPMC1289132en_US
dc.identifier.urihttp://hdl.handle.net/10342/3340en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://www.cell.com/biophysj/en_US
dc.rightsAuthor notified of opt-out rights by Cammie Jennings prior to upload of this article.en_US
dc.subjectCalponinen_US
dc.subjectAlpha-actininen_US
dc.subjectActinen_US
dc.titleCalponin interaction with alpha-actinin-actin: evidence for a structural role for calponin.en_US
dc.typeArticleen_US
ecu.journal.issue6
ecu.journal.nameBiophysical Journal
ecu.journal.pages3208-3217
ecu.journal.volume77

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