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HuR binds to a single site on the C/EBP - beta mRNA of 3T3-L1 Adipocytes

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dc.contributor.authorJones, Heathen_US
dc.contributor.authorCarver, Melindaen_US
dc.contributor.authorPekala, Phillip H.en_US
dc.date.accessioned2011-04-13T20:53:41Zen_US
dc.date.accessioned2011-05-17T01:27:02Z
dc.date.available2011-04-13T20:53:41Zen_US
dc.date.available2011-05-17T01:27:02Z
dc.date.issued2007-03-30en_US
dc.description.abstractHuR is a ligand for nuclear mRNAs containing adenylate-uridylate rich elements in the 3'- untranslated region. Once bound to the mRNA, HuR is recognized by adapter proteins which then facilitate nuclear export of the complex. In the cytosol HuR is thought to function to control stability and translation of its ligand message. In the 3T3-L1 cells HuR is constitutively expressed and localized predominantly to the nucleus in the preadipocytes. However within 30 min of exposure to the differentiation stimulus, the HuR content in the cytosol increases consistent with HuR regulating the availability of relevant mRNAs for translation. Using in vitro RNA gel shifts, we have demonstrated that the C/EBPß message is a ligand for HuR and that the single binding site is an adenylate-uridylate rich element in the 3'untranslated region. Originally published Biochemical and Biophysical Research Communications, Vol. 355, No. 1, Mar 2007en_US
dc.identifier.citationBiochemical and Biophysical Research Communications; 355:1 p. 217-220en_US
dc.identifier.doi10.1016/j.bbrc.2007.01.136
dc.identifier.pmidPMC1839008en_US
dc.identifier.urihttp://hdl.handle.net/10342/3315en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4MYMJJF-8&_user=10&_coverDate=03%2F30%2F2007&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=41beeded65d947ad9b1f39f98e5c276f&searchtype=aen_US
dc.rightsAuthor notified of opt-out rights by Cammie Jennings prior to upload of this article.en_US
dc.subjectCCAAT enhancer binding protein betaen_US
dc.subjectHuRen_US
dc.subjectElaven_US
dc.subject3T3-L1en_US
dc.subjectAdipocytesen_US
dc.subjectDifferentiationen_US
dc.titleHuR binds to a single site on the C/EBP - beta mRNA of 3T3-L1 Adipocytesen_US
dc.typeArticleen_US
ecu.journal.issue1
ecu.journal.nameBiochemical and Biophysical Research Communications
ecu.journal.pages217-220
ecu.journal.volume355

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