Evidence for the presence of A1 adenosine receptors in the aorta of spontaneously hypertensive rats
dc.contributor.author | Fahim, Mohammed | en_US |
dc.contributor.author | Mustafa, Syed Jamal | en_US |
dc.date.accessioned | 2011-04-29T13:06:38Z | en_US |
dc.date.accessioned | 2011-05-17T13:10:57Z | |
dc.date.available | 2011-04-29T13:06:38Z | en_US |
dc.date.available | 2011-05-17T13:10:57Z | |
dc.date.issued | 2001-12 | en_US |
dc.description.abstract | 1 Isolated aortic rings (endothelium-intact and -denuded) from spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats were used in this study to examine the vasoactive e ects of various adenosine analogues. 2 In phenylephrine contracted aortic rings, concentration-response curves were constructed by cumulative additions (10711 ±1075 M) of (2S)-N6-[2-endo-Norbornyl] adenosine (ENBA), N6- cyclopentyladenosine (CPA), R-N6-(2-phenylisopropyl) adenosine (R-PIA), 2-p-(-2-carboxyethyl) phenethylamino-5'-N-thylcarboxamido adenosine (CGS-21680). 3 A non-speci®c adenosine receptor agonist 2-chloroadenosine (CAD) resulted in biphasic response with a small contraction at lower concentrations (1079 ±1078 M) followed by a signi®cant relaxation at higher concentration in endothelium-intact SHR tissues, suggesting presence of both A1 and A2 adenosine receptors in SHR aorta. However, only relaxation was observed in WKY. 4 Contractile response in SHR had the following rank order of potency: ENBA4CPA4R- PIA4CAD. The relaxation response in SHR and WKY had the following rank order of potency: CGS 216804CAD4R-PIA4CPA4ENBA. 5 Removal of endothelium abolished the adenosine analogue induced contractions in SHR aorta and attenuated the vasorelaxation responses in the WKY and SHR. 6 The contractile response in SHR was abolished by A1 adenosine receptor antagonist N6- endonorbornan-2-yl-9-methyladenine (N-0861). A2 adenosine receptor antagonist, 3,7-dimethyl-1-proparglyxanthine (DMPX) did not a ect the contraction response of adenosine analogues. 7 Endothelium-dependent contractions elicited by A1 receptor agonists were blocked by indomethacin and by free radical scavengers. 8 These data suggest that the contractile response to adenosine analogues in SHR aorta is probably mediated by free radicals which are generated through the increased cyclo-oxygenase activity occurring in the vascular endothelium of SHR but not the WKY rats. British Journal of Pharmacology (2001) 134, 1760 ± 1766. Originally published British Journal of Pharmacology, Vol. 134, No. 8, Dec 2001 | en_US |
dc.identifier.citation | British Journal of Pharmacology; 134:8 p. 1760-1766 | en_US |
dc.identifier.doi | 10.1038/sj.bjp.0704433 | |
dc.identifier.pmid | PMC1572910 | en_US |
dc.identifier.uri | http://hdl.handle.net/10342/3441 | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | East Carolina University | en_US |
dc.relation.uri | http://www.brjpharmacol.org/view/0/index.html | en_US |
dc.rights | Author notified of opt-out rights by Cammie Jennings prior to upload of this article. | en_US |
dc.subject | A1 adenosine receptor | en_US |
dc.subject | A2 adenosine receptor | en_US |
dc.subject | Vascular endothelium | en_US |
dc.subject | Vascular smooth muscle | en_US |
dc.subject | Indomethacin | en_US |
dc.subject | Free radicals | en_US |
dc.title | Evidence for the presence of A1 adenosine receptors in the aorta of spontaneously hypertensive rats | en_US |
dc.type | Article | en_US |
ecu.journal.issue | 8 | |
ecu.journal.name | British Journal of Pharmacology | |
ecu.journal.pages | 1760-1766 | |
ecu.journal.volume | 134 |
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